investigation of the intracellular site of anthocyanoplasts using isolated protoplasts and vacuoles

• Published in: Plant cell reports Vol. 7; no. 6; pp. 389 - 392
• Main Authors: Nozzolillo, C, Ishikura, N
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-10-1988
• Subjects: anthocyanins; Biological and medical sciences; Brassica oleracea; Cell physiology; Cells, cell elements: structure and function; Fundamental and applied biological sciences. Psychology; isolation; plant anatomy; Plant physiology and development; protoplasts; Prunus; Prunus yedoensis; Raphanus sativus; vacuoles; Vigna radiata; Anthocyanin; Rosaceae; Vigna radiata; Vacuole; Raphanus sativus; Food crop; Optical microscopy; Brassica oleracea; Leguminosae; Vegetable crop; Cruciferae; Fruit crop; Dicotyledones; Protoplast; Angiospermae; Prunus; Spermatophyta; Intracellular; Fodder crop; Localization
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/bf00269519

Microscopic observations made during preparation of protoplasts and vacuoles from red radish seedling hypocotyl (Raphanus sativus L.) show that anthocyanoplasts, the strongly pigmented bodies present in the pigmented cells of the hypodermis, begin as apparently membranous vesicles in the cytoplasm made visible by the deposition and accumulation of anthocyanins, but only rarely appear in the isolated vacuole. Isolation of protoplasts and vacuoles was also achieved from mung bean seedling hypocotyl (Vigna radiata L Wilczek), red cabbage leaf (Brassica oleracea L.) and Prunus x yedoensis Matsum callus. Anthocyanoplasts were usually in the vacuole, although sometimes in the cytoplasm, of the mung bean and cabbage, but were never seen in vacuoles of Prunus callus.