A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation

Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy Power...

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Bibliographic Details
Published in:	Separation science and technology Vol. 54; no. 18; pp. 3128 - 3134
Main Authors:	Suzuki, Yoshihiro, Imafuku, Yuki, Nishiyama, Masateru, Teranishi, Kotaro, Jikumaru, Atsushi, Nukazawa, Kei, Ogura, Yoshitoshi
Format:	Journal Article
Language:	English
Published:	Abingdon Taylor & Francis 12-12-2019 Taylor & Francis Ltd
Subjects:	16S rRNA gene sequencing analysis Bacteria Bacteria DNA Casein Coagulation Deoxyribonucleic acid DNA DNA sequencing E coli Ferric chloride Flora foam concentration Foam separation Gene sequencing Iron chlorides Milk Plants River water Rivers rRNA 16S Separation Test procedures
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Summary:	Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67-2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.
ISSN:	0149-6395 1520-5754
DOI:	10.1080/01496395.2019.1565774