Simultaneous Determination of Sudan Dyes and Carotenoids in Red Pepper and Tomato Products by HPLC

• Published in: Journal of chromatographic science Vol. 43; no. 9; pp. 461 - 465
• Main Authors: Daood, Hussein G., Biacs, Péter A.
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01-10-2005; Preston Publications
• Subjects: Aroma and flavouring agent industries; Biological and medical sciences; Carotenoids - analysis; Chromatography, High Pressure Liquid - methods; Coloring Agents - analysis; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; General pharmacology; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Vegetables - chemistry; Validation; Elution; Gradient; Chemical analysis; Pharmacognosy; HPLC chromatography; Medicinal plant; Vegetable crop; Dicotyledones; Azo dye; Angiospermae; Simultaneous measurement; Capsicum annuum; Flavoring crop; Diode array; Spermatophyta; Solanaceae; Tomato concentrate; Carotenoid; Chilli pepper; Quantitative analysis; Ultraviolet visible spectrometry
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/43.9.461

To simultaneously detect Sudan dyes and carotenoids in red pepper and tomato products, high-performance liquid chromatography (HPLC) methods with photodiode-array detection are developed and validated. The methods include the use of end-capped and nonend-capped adsorbents with a gradient elution system starting with water containing methanol. Water content of 9% in the starting mobile phase is found to be necessary to ensure sufficient separation of Sudan dyes and to avoid overlapping or interference with the carotenoids of considerable content. The data of the validation reveal the accuracy and precision of the developed methods. A limit of Sudan dyes detection of 1–5 µg/g in red pepper or tomato sauce could be approached. The methods provide excellent separation of the carotenoids from the unsaponified extracts of red pepper and the tomato products