Lipid mediator profiles of burn wound healing: Acellular cod fish skin grafts promote the formation of EPA and DHA derived lipid mediators following seven days of treatment

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 175; p. 102358
• Main Authors: Kotronoulas, Aristotelis, de Lomana, Adrián López García, Karvelsson, Sigurður Trausti, Heijink, Marieke, Stone II, Randolph, Giera, Martin, Rolfsson, Ottar
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01-12-2021
• Subjects: Animals; Biomaterials; Burns - etiology; Burns - metabolism; Burns - therapy; Cattle; Chromatography, High Pressure Liquid; Disease Models, Animal; Docosahexaenoic Acids - metabolism; Eicosanoids; Eicosapentaenoic Acid - metabolism; Gadiformes; Inflammmation; Lipid biochemistry; Lipid Metabolism; Lipidomics - methods; Mass spectrometry; Omega-3 fatty acids; Phosphatidylcholines - metabolism; Skin Transplantation - methods; Swine; Tandem Mass Spectrometry; Wound Healing; AA; NSI; SPMs; Eicosanoids; KETE; DPT; FT; Biomaterials; HETE; ω3-FA; Inflammmation; LA; MeOH; RvD1; Lipid biochemistry; EPA; FSG; DHA; Mass spectrometry; Omega-3 fatty acids
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/j.plefa.2021.102358

•This is the first study to investigate whether omega-3 rich biomaterial such as fish skin graft can affect the lipidome of burn wound healing.•The study monitors the formation of lipid mediators during 60 days of burn wound healing.•This is the first study to show an increase in the formation of mono-hydroxylated derivatives of EPA and DHA after seven days of treatment with fish skin graft.•The study suggests that FSG may alter the amounts of EPA and DHA lipid mediators during the early burn wound healing.
The use of acellular fish skin grafts (FSG) for the treatment of burn wounds is becoming more common due to its beneficial wound healing properties. In our previous study we demonstarted that FSG is a scaffold biomaterial that is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) conjugated to phosphatidylcholines. Here we investigated whether EPA and DHA derived lipid mediators are influenced during the healing of burn wounds treated with FSG. Deep partial and full thickness burn wounds (DPT and FT, respectively) were created on Yorkshire pigs (n = 4). DPT were treated with either FSG or fetal bovine dermis while FT were treated either with FSG or cadaver skin initially and followed by a split thickness skin graft. Punch biopsies were collected on days 7, 14, 21, 28 and 60 and analyzed in respect of changes to approximately 45 derivatives of EPA, DHA, arachidonic acid (AA), and linoleic acid (LA) employing UPLC-MS/MS methodology. Nine EPA and DHA lipid mediators, principally mono-hydroxylated derivatives such as 18-HEPE and 17-HDHA, were significantly higher on day 7 in the DPT when treated with FSG. A similar but non-significant trend was observed for the FT. The results suggest that the use of FSG in burn wound treatment can alter the formation of EPA and DHA mono hydroxylated lipid mediators in comparison to other grafts of mammalian origin. The differences observed during the first seven days after treatment indicates that FSG affects the early stages of wound healing.