Human papillomavirus genotypes distribution in cervical samples from women living with human immunodeficiency virus

• Published in: Archives of gynecology and obstetrics Vol. 283; no. 4; pp. 809 - 817
• Main Authors: Melgaço, Fabiana G., Rosa, Maria L. G., Augusto, Everton F., Haimuri, Jacqueline G. S., Jacintho, Claudia, Santos, Larissa S., Cavalcanti, Silvia M. B., Oliveira, Ledy H. S.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-04-2011; Springer Nature B.V
• Subjects: Adolescent; Adult; Alphapapillomavirus - genetics; Brazil - epidemiology; Cervical cancer; Cervix Uteri - virology; Cohort analysis; Comorbidity; Cross-Sectional Studies; Endocrinology; Female; General Gynecology; Genotype; Gynecology; HIV; HIV Infections - complications; HIV Infections - epidemiology; Human Genetics; Human immunodeficiency virus; Human papillomavirus; Humans; Medicine; Medicine & Public Health; Middle Aged; Obstetrics/Perinatology/Midwifery; Papillomavirus Infections - complications; Papillomavirus Infections - epidemiology; Vaginal Smears; Young Adult; Brazil; Human papillomavirus; Restriction fragment length polymorphism; Human immunodeficiency virus; Cohort study
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-010-1443-z

Background
The aim of this study was to determine the prevalence and relationship of human papillomavirus (HPV) genotypes in cervical samples from 140 human immunodeficiency virus (HIV)-seropositive women routinely attending an outpatient public gynecological service in the state of Rio de Janeiro, Brazil.
Methods
MY09/11 consensus primers were used to detect generic HPV DNA. HPV typification was performed by restriction fragment length polymorphism analysis following polymerase chain reaction amplification.
Results
The overall HPV prevalence was 60.0%. The identification of 24 different HPV genotypes including uncommon types was performed. A9 oncogenic genotypes were present in 54.02% of HIV-positive women. Abnormal cervical lesions, the time since HIV diagnosis and the number of sexual partners contributed independently to the high oncogenic HPV prevalence. The oncogenic and non-oncogenic group were similarly affected by risk factors in contrast to negative HPV women. The frequency of multiple infections was 20%; furthermore, all of them presented at least one oncogenic type.
Conclusion
The analyzed sample represents an epidemiological source of uncommon infection. Due to high HPV prevalence, more frequent cytological screening and/or liberal colposcopic evaluations should be performed in HIV-positive patients.