Association of ionophores, yeast, and bacterial probiotics alters the abundance of ruminal microbial species of pasture intensively finished beef cattle

• Published in: Tropical animal health and production Vol. 53; no. 1; p. 172
• Main Authors: Mombach, Mircéia Angele, da Silva Cabral, Luciano, Lima, Leni Rodrigues, Ferreira, Daniela Cristina, Pedreira, Bruno Carneiro e, Pereira, Dalton Henrique
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 17-02-2021; Springer Nature B.V
• Subjects: Additives; Animal diseases; Animal Feed - analysis; Animals; Archaea; Bacteria; Beef; Beef cattle; Biomedical and Life Sciences; Body weight; Cattle; Diet; Diet - veterinary; Feed additives; Fermentation; Fibrobacter; High protein diet; Ionophores; Life Sciences; Metabolic disorders; Microbiomes; Microorganisms; Nitrogen; Pasture; Probiotics; Proteins; Protozoa; Regular Articles; Rumen - metabolism; Ruminococcus; Saccharomyces cerevisiae; Salinomycin; Urea; Veterinary Medicine/Veterinary Science; Virginiamycin; Yeast; Yeasts; Zoology; High-concentrate diets; Slow-release urea; Real-time PCR; Fibrolytic bacteria; Rumen microbiology
• DOI: 10.1007/s11250-021-02617-2
• EISSN: 1573-7438
• ISSN: 0049-4747

The effect of the association of non-protein nitrogen, yeast, and bacterial probiotics on the ruminal microbiome of beef cattle intensively finished on pasture was evaluated. The experiment was carried out in a completely randomized design with five treatments and four replications. The treatments consisted of a group of animals kept on pasture that received low consumption supplementation (LS) and four groups that received for 98 days, 17.5 g concentrate kg
−1
body weight. The supplements were composed of the association of additives: urea (U), slow-release non-protein nitrogen (U+SRN), yeast (
Saccharomyces cerevisiae
; U+SRN+Y), and bacterial probiotics (live strains of bacteria; U+SRN+Y+BP). All supplements also contained salinomycin and virginiamycin. After slaughtering the animals, samples of ruminal content were collected to quantify groups of fibrolytic bacteria (
Ruminococcus albus
and
Fibrobacter succinogenes
), non-fibrolytic (
Prevotella ruminicola, Selenomonas ruminantium
, and
Streptococcus bovis
), Archaea, and ciliate protozoa, using the qPCR technique. The abundance of
F. succinogenes
was the same for the LS animals and those that received the supplement U+SRN+Y (1.42×10
8
copies mL
−1
) but higher than the other treatments. Supplementation reduced by 90% the abundance of
S. bovis
compared to the LS. The inclusion of yeast increased the abundance of fibrolytic bacteria by 2.2-fold. For animals that received the supplement U+SRN+Y+BP and the LS, there was no difference for non-fibrolytic bacteria (3.07×10
9
copies mL
−1
). The use of yeasts and sources of non-protein nitrogen in high-concentrate diets for beef cattle stimulates the growth of fibrolytic bacteria, which can contribute to the reduction of digestive disorders and metabolic diseases in animals that receive diets with high concentrate in pasture intensive termination systems.