Polymorphisms of the CYP1A1 and CYP2E1 genes in head and neck squamous cell carcinoma risk

• Published in: Molecular biology reports Vol. 39; no. 2; pp. 1055 - 1063
• Main Authors: Cury, Nathália Moreno, Russo, Anelise, Galbiatti, Ana Lívia Silva, Ruiz, Mariângela Torreglosa, Raposo, Luiz Sérgio, Maniglia, José Victor, Pavarino, Érika Cristina, Goloni-Bertollo, Eny Maria
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-02-2012; Springer Nature B.V
• Subjects: Age; Age Factors; Alcohol Drinking; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Carcinogens; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - genetics; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP2E1 - genetics; Cytochrome P450; Cytochromes; Enzymes; Ethanol; Gene polymorphism; Genetic Association Studies; Genetic Predisposition to Disease - genetics; Head & neck cancer; Head and neck cancer; Head and Neck Neoplasms - epidemiology; Head and Neck Neoplasms - genetics; Histology; Humans; Larynx; Life Sciences; Logistic Models; Molecular biology; Morphology; Odds Ratio; Polymerase Chain Reaction; Polymorphism; Polymorphism, Genetic - genetics; Polymorphism, Restriction Fragment Length; Restriction fragment length polymorphism; Risk Factors; Smoking; squamous cell carcinoma; Statistical analysis; Head and neck squamous cell carcinoma; genes; and; Polymorphism
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-0831-1

Polymorphisms in genes that encode P450 cytochrome enzymes may increase carcinogen activation or decrease their inactivation and consequently, promote the development of cancer. The aims of this study were to identify the
Msp
I-
CYP1A1
,
Pst
I-
CYP2E1
and
Dra
I-
CYP2E1
polymorphisms in patients with head and neck cancer and to compare with individuals without cancer; to evaluate the association of these polymorphisms with risk factors and clinical histopathological parameters. In the study group, 313 patients were evaluated for
CYP1A1
, 217 for
CYP2E1
(
Pst
I) and 211 for
CYP2E1
(
Dra
I) and in the control group 417, 334 and 374 individuals, respectively
.
Molecular analysis was performed by PCR–RFLP technique, and chi-square and multiple logistic regression tests were used for statistical analysis. The result of analysis regarding individuals evaluated for
CYP1A1
(
Msp
I) showed that age (OR: 8.15; 95% CI 5.57–11.92) and smoking (OR: 5.37; 95% CI 3.52–8.21) were predictors for the disease; for the
CYP2E1
(
Pst
I and
Dra
I), there were associations with age (
Pst
I-OR: 9.10; 95% CI 5.86–14.14/
Dra
I-OR: 8.07; 95% CI 5.12–12.72), smoking (
Pst
I-OR: 4.10; 95% CI 2.44–6.89/
Dra
I-OR: 5.73; 95% CI 3.34–9.82), alcohol (
Pst
I-OR: 1.93; 95% CI 1.18–3.16/
Dra
I-OR: 1.69; 95% CI 1.02–2.81), respectively, with disease development.
CYP2E1
(
Pst
I) was less frequent in patient group (OR: 0.48; 95% CI 0.23–0.98). Regarding clinical histopathological parameters,
CYP1A1
polymorphism was less frequent in the larynx primary anatomic site (OR = 0.45; 95% CI = 0.28–0.73;
P
 = 0.014). In conclusion, we confirm that age, smoking and alcohol consumption are risk factors for this disease and the polymorphisms investigated have no association with the development of head and neck cancer.