High-level secretion of hirudin by Hansenula polymorpha--authentic processing of three different preprohirudins

A DNA sequence coding for a subtype of the hirudin variant HV1 was expressed in the methylotrophic yeast Hansenula polymorpha from a strongly inducible promoter element derived from a gene of the methanol metabolism pathway. For secretion, the coding sequence was fused to the KEX2 recognition site o...

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Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 44; no. 3/4; pp. 377 - 385
Main Authors:	Weydemann, U, Keup, P, Piontek, M, Strasser, A.W.M, Schweden, J, Gellissen, G, Janowicz, Z.A
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-12-1995
Subjects:	Amino Acid Sequence Animals Arthropod Proteins Biological and medical sciences Biological Sciences Biotechnology Brachyura - genetics Fermentation Fundamental and applied biological sciences. Psychology Fungal Proteins - biosynthesis Fungal Proteins - genetics Genetic engineering Genetic technics Glucan 1,4-alpha-Glucosidase - biosynthesis Glucan 1,4-alpha-Glucosidase - genetics Hansenula polymorpha Hirudins - biosynthesis Hirudins - genetics Hirudins - metabolism Invertebrate Hormones - biosynthesis Invertebrate Hormones - genetics Mating Factor Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Peptide Biosynthesis Peptides - genetics Pichia - metabolism Promoter Regions, Genetic Protein Precursors - metabolism Protein Processing, Post-Translational Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - genetics Recombinant microorganism Yeast Molecular processing Leader sequence Secretion Gene expression Fermentation Precursor Fungi Plasmid Ascomycetes Recombinant protein Hansenula polymorpha Hybrid gene Thallophyta
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