Site-Specific Lipidation Enhances IFITM3 Membrane Interactions and Antiviral Activity
Interferon-induced transmembrane proteins (IFITMs) are -palmitoylated proteins in vertebrates that restrict a diverse range of viruses. -palmitoylated IFITM3 in particular engages incoming virus particles, prevents their cytoplasmic entry, and accelerates their lysosomal clearance by host cells. How...
Saved in:
| Published in: | ACS chemical biology Vol. 16; no. 5; pp. 844 - 856 |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
21-05-2021
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Interferon-induced transmembrane proteins (IFITMs) are
-palmitoylated proteins in vertebrates that restrict a diverse range of viruses.
-palmitoylated IFITM3 in particular engages incoming virus particles, prevents their cytoplasmic entry, and accelerates their lysosomal clearance by host cells. However, how
-palmitoylation modulates the structure and biophysical characteristics of IFITM3 to promote its antiviral activity remains unclear. To investigate how site-specific
-palmitoylation controls IFITM3 antiviral activity, we employed computational, chemical, and biophysical approaches to demonstrate that site-specific lipidation of cysteine 72 enhances the antiviral activity of IFITM3 by modulating its conformation and interaction with lipid membranes. Collectively, our results demonstrate that site-specific
-palmitoylation of IFITM3 directly alters its biophysical properties and activity in cells to prevent virus infection. |
|---|---|
| AbstractList | Interferon-induced transmembrane proteins (IFITMs) are
S
-palmitoylated proteins in vertebrates that restrict a diverse range of viruses.
S
-palmitoylated IFITM3 in particular engages incoming virus particles, prevents their cytoplasmic entry, and accelerates their lysosomal clearance by host cells. However, how
S
-palmitoylation modulates the structure and biophysical characteristics of IFITM3 to promote its antiviral activity remains unclear. To investigate how site-specific
S
-palmitoylation controls IFITM3 antiviral activity, we employed computational, chemical, and biophysical approaches to demonstrate that site-specific lipidation of cysteine 72 enhances the antiviral activity of IFITM3 by modulating its conformation and interaction with lipid membranes. Collectively, our results demonstrate that site-specific
S
-palmitoylation of IFITM3 directly alters its biophysical properties and activity in cells to prevent virus infection. Interferon-induced transmembrane proteins (IFITMs) are -palmitoylated proteins in vertebrates that restrict a diverse range of viruses. -palmitoylated IFITM3 in particular engages incoming virus particles, prevents their cytoplasmic entry, and accelerates their lysosomal clearance by host cells. However, how -palmitoylation modulates the structure and biophysical characteristics of IFITM3 to promote its antiviral activity remains unclear. To investigate how site-specific -palmitoylation controls IFITM3 antiviral activity, we employed computational, chemical, and biophysical approaches to demonstrate that site-specific lipidation of cysteine 72 enhances the antiviral activity of IFITM3 by modulating its conformation and interaction with lipid membranes. Collectively, our results demonstrate that site-specific -palmitoylation of IFITM3 directly alters its biophysical properties and activity in cells to prevent virus infection. |
| Author | Im, Wonpil Percher, Avital Li, Yumeng Bhattacharya, Shibani Peng, Tao Lee, Hwayoung Das, Tandrila Wiewiora, Rafal Witte, Isaac P Garst, Emma H Hang, Howard C |
| AuthorAffiliation | 1 Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, United States 2 Tri-Institutional Ph.D. Program in Chemical Biology, New York, NY 10065, United States 5 Memorial Sloan Kettering Cancer Center, New York, NY 10065, United States 7 Departments of Immunology and Microbiology and Chemistry, Scripps Research, La Jolla, CA 92037, United States 6 State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China 3 Department of Biological Sciences, Chemistry, and Bioengineering, Lehigh University, Bethlehem, PA 18015, United States 4 New York Structural Biology Center, New York, NY 10027, United States |
| AuthorAffiliation_xml | – name: 2 Tri-Institutional Ph.D. Program in Chemical Biology, New York, NY 10065, United States – name: 6 State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China – name: 7 Departments of Immunology and Microbiology and Chemistry, Scripps Research, La Jolla, CA 92037, United States – name: 1 Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, United States – name: 4 New York Structural Biology Center, New York, NY 10027, United States – name: 3 Department of Biological Sciences, Chemistry, and Bioengineering, Lehigh University, Bethlehem, PA 18015, United States – name: 5 Memorial Sloan Kettering Cancer Center, New York, NY 10065, United States |
| Author_xml | – sequence: 1 givenname: Emma H orcidid: 0000-0001-8170-2343 surname: Garst fullname: Garst, Emma H organization: Tri-Institutional Ph.D. Program in Chemical Biology, New York, New York 10065, United States – sequence: 2 givenname: Hwayoung orcidid: 0000-0003-2332-1993 surname: Lee fullname: Lee, Hwayoung organization: Department of Biological Sciences, Chemistry, and Bioengineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States – sequence: 3 givenname: Tandrila surname: Das fullname: Das, Tandrila organization: Tri-Institutional Ph.D. Program in Chemical Biology, New York, New York 10065, United States – sequence: 4 givenname: Shibani surname: Bhattacharya fullname: Bhattacharya, Shibani organization: New York Structural Biology Center, New York, New York 10027, United States – sequence: 5 givenname: Avital surname: Percher fullname: Percher, Avital organization: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, United States – sequence: 6 givenname: Rafal orcidid: 0000-0002-8961-7183 surname: Wiewiora fullname: Wiewiora, Rafal organization: Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States – sequence: 7 givenname: Isaac P surname: Witte fullname: Witte, Isaac P organization: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, United States – sequence: 8 givenname: Yumeng surname: Li fullname: Li, Yumeng organization: State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China – sequence: 9 givenname: Tao orcidid: 0000-0002-5578-5314 surname: Peng fullname: Peng, Tao organization: State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China – sequence: 10 givenname: Wonpil orcidid: 0000-0001-5642-6041 surname: Im fullname: Im, Wonpil organization: Department of Biological Sciences, Chemistry, and Bioengineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States – sequence: 11 givenname: Howard C orcidid: 0000-0003-4053-5547 surname: Hang fullname: Hang, Howard C organization: Departments of Immunology and Microbiology and Chemistry, Scripps Research, La Jolla, California 92037, United States |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33887136$$D View this record in MEDLINE/PubMed |
| BookMark | eNpVkM1uwjAQhK2Kqvy0L9BD5RcI9dr586USQtBGAvUAnK2N4xRX4ERxisTbNwhK29OOtDszq29Ieq5yhpBHYGNgHJ5Re701-9xWY9CMMRA3ZABRFAapFEnvqrnsk6H3n4yFIk7lHekLkaYJiHhANivbmmBVG21Lq-nC1rbA1laOztwWnTaeZvNsvRR02TU16AzNXGsa1KcjT9EVdOJae7AN7uhEn1R7vCe3Je68ebjMEdnMZ-vpW7B4f82mk0WgQ8bbgGOOhgMWkplQJKZMOeNG6jwOYxnHOSaFRiiTEhkTAkuZA0CSyFAUeSRKECPycs6tv_K9KbRxbfeGqhu7x-aoKrTq_8bZrfqoDkoC8DiSXQA_B-im8r4x5dULTJ0gq1_I6gK5Mz39bb1afqiKb5Yifv8 |
| CitedBy_id | crossref_primary_10_3390_pharmaceutics14112533 crossref_primary_10_3390_v16020254 crossref_primary_10_3390_v16050734 crossref_primary_10_1002_pro_4791 crossref_primary_10_1021_acs_chemrev_3c00850 crossref_primary_10_1021_acs_chemrev_4c00112 crossref_primary_10_3389_fimmu_2022_1042368 crossref_primary_10_3390_v15122329 crossref_primary_10_1016_j_jmb_2021_167424 crossref_primary_10_1016_j_jmb_2021_167438 crossref_primary_10_1021_acschembio_2c00176 crossref_primary_10_1038_s41577_021_00626_8 crossref_primary_10_1002_pro_4618 crossref_primary_10_1016_j_sbi_2022_102467 crossref_primary_10_1016_j_chom_2023_03_005 crossref_primary_10_1016_j_molcel_2023_09_004 crossref_primary_10_1128_mbio_02289_22 crossref_primary_10_1016_j_cbpa_2021_06_004 crossref_primary_10_3389_fimmu_2022_844657 crossref_primary_10_3389_fimmu_2022_919477 |
| Cites_doi | 10.15252/embj.2020106267 10.1074/jbc.M113.514356 10.1038/s41586-020-2884-6 10.1080/22221751.2020.1787797 10.1371/journal.ppat.1007532 10.1093/infdis/jix512 10.15252/embj.2020106501 10.1016/j.cell.2017.11.018 10.1074/jbc.M114.557694 10.1021/cr2001977 10.1074/jbc.AC119.010611 10.1093/nar/gky995 10.1146/annurev-virology-031413-085537 10.1093/infdis/jix242 10.1016/j.celrep.2016.05.074 10.1038/nchembio.405 10.1002/eji.201847692 10.1073/pnas.0710389105 10.1038/nchembio.388 10.1021/ja054842f 10.1038/nrm2084 10.1146/annurev.biochem.052308.105824 10.1074/jbc.M112.362095 10.1146/annurev-biochem-060713-035737 10.1016/j.chembiol.2020.03.004 10.1021/acsnano.0c10567 10.1371/journal.ppat.1004048 10.1021/acs.jproteome.8b00002 10.1038/nature10921 10.1083/jcb.200404100 10.1016/j.cell.2009.12.017 10.1128/JVI.00481-13 10.1016/j.jmb.2013.09.024 10.1038/nchembio834 10.1128/JVI.01254-16 10.1039/D0CC06073A 10.2217/fmb.14.65 10.1371/journal.ppat.1001258 10.1021/jacs.6b08733 10.1074/jbc.M117.800482 10.1073/pnas.1900784116 10.1073/pnas.1602244113 10.1016/j.cbpa.2013.07.031 10.15252/embr.201642771 10.1371/journal.pone.0104341 10.1093/infdis/jiaa224 10.1038/nature09907 10.1007/s10858-009-9333-z 10.1083/jcb.200507067 10.1038/srep24029 10.1016/j.cell.2010.04.007 10.1074/jbc.M110.173583 10.1002/anie.201707554 10.1038/s41589-018-0213-2 10.1038/s41586-020-2355-0 10.1038/nm.4370 10.1126/science.1105654 10.1371/journal.pone.0143539 10.1073/pnas.1016184107 10.1021/cr400355w 10.4049/jimmunol.1201305 10.1016/j.cbpa.2013.08.004 10.15252/embr.201744100 10.1002/anie.201914836 10.1016/j.chom.2013.03.006 10.26508/lsa.201900542 10.1038/nchembio0605-13 10.1038/ni.2525 10.1038/emboj.2012.15 10.1016/0092-8674(84)90270-8 10.1126/science.aaw7733 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 5PM |
| DOI | 10.1021/acschembio.1c00013 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| EISSN | 1554-8937 |
| EndPage | 856 |
| ExternalDocumentID | 10_1021_acschembio_1c00013 33887136 |
| Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: T32 GM115327 – fundername: NCI NIH HHS grantid: P30 CA008748 – fundername: NIGMS NIH HHS grantid: R01 GM087544 – fundername: NIGMS NIH HHS grantid: P41 GM066354 – fundername: NIAID NIH HHS grantid: R21 AI156674 – fundername: NCRR NIH HHS grantid: C06 RR015495 |
| GroupedDBID | --- 23M 4.4 53G 55A 5GY 5VS 5ZA 6J9 7~N AABXI ABJNI ABMVS ABQRX ABUCX ACGFO ACGFS ACS ADHLV AEESW AENEX AFEFF AHGAQ ALMA_UNASSIGNED_HOLDINGS AQSVZ BAANH CGR CS3 CUPRZ CUY CVF D0L EBS ECM ED~ EIF F5P GGK GNL GX1 IH9 IHE JG~ NPM P2P RNS ROL UI2 VF5 VG9 W1F AAYXX CITATION 5PM |
| ID | FETCH-LOGICAL-c402t-2abae21ad90e437ef8202e9cb646966ba7dca1f7fa0033af9b11177943db53f13 |
| IEDL.DBID | ACS |
| ISSN | 1554-8929 |
| IngestDate | Tue Sep 17 21:04:44 EDT 2024 Fri Aug 23 02:58:26 EDT 2024 Sat Sep 28 08:20:14 EDT 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c402t-2abae21ad90e437ef8202e9cb646966ba7dca1f7fa0033af9b11177943db53f13 |
| Notes | These authors contributed equally. |
| ORCID | 0000-0002-5578-5314 0000-0001-8170-2343 0000-0003-4053-5547 0000-0001-5642-6041 0000-0003-2332-1993 0000-0002-8961-7183 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112659 |
| PMID | 33887136 |
| PageCount | 13 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_9112659 crossref_primary_10_1021_acschembio_1c00013 pubmed_primary_33887136 |
| PublicationCentury | 2000 |
| PublicationDate | 2021-05-21 |
| PublicationDateYYYYMMDD | 2021-05-21 |
| PublicationDate_xml | – month: 05 year: 2021 text: 2021-05-21 day: 21 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | ACS chemical biology |
| PublicationTitleAlternate | ACS Chem Biol |
| PublicationYear | 2021 |
| References | ref9/cit9 ref45/cit45 ref3/cit3 ref27/cit27 ref63/cit63 Prelli Bozzo C. (ref74/cit74) 2020 ref56/cit56 ref16/cit16 ref52/cit52 ref23/cit23 ref8/cit8 ref31/cit31 ref59/cit59 ref2/cit2 ref34/cit34 ref71/cit71 ref37/cit37 ref20/cit20 ref48/cit48 ref60/cit60 ref17/cit17 Buchrieser J. (ref19/cit19) 2019; 365 ref10/cit10 ref35/cit35 ref53/cit53 ref21/cit21 ref42/cit42 ref46/cit46 ref49/cit49 ref13/cit13 ref61/cit61 ref67/cit67 ref24/cit24 ref38/cit38 ref50/cit50 ref64/cit64 ref54/cit54 ref6/cit6 ref36/cit36 ref18/cit18 ref65/cit65 ref11/cit11 ref25/cit25 ref72/cit72 ref32/cit32 ref39/cit39 ref14/cit14 ref57/cit57 ref5/cit5 ref51/cit51 ref43/cit43 ref28/cit28 ref40/cit40 ref68/cit68 ref26/cit26 Zang R. (ref29/cit29) 2020; 30 ref55/cit55 ref73/cit73 ref69/cit69 ref12/cit12 ref15/cit15 ref62/cit62 ref66/cit66 ref41/cit41 ref58/cit58 ref22/cit22 ref33/cit33 ref4/cit4 ref30/cit30 ref47/cit47 ref1/cit1 ref44/cit44 ref70/cit70 ref7/cit7 |
| References_xml | – ident: ref27/cit27 doi: 10.15252/embj.2020106267 – ident: ref38/cit38 doi: 10.1074/jbc.M113.514356 – ident: ref17/cit17 doi: 10.1038/s41586-020-2884-6 – ident: ref28/cit28 doi: 10.1080/22221751.2020.1787797 – ident: ref34/cit34 doi: 10.1371/journal.ppat.1007532 – ident: ref23/cit23 doi: 10.1093/infdis/jix512 – ident: ref73/cit73 doi: 10.15252/embj.2020106501 – ident: ref13/cit13 doi: 10.1016/j.cell.2017.11.018 – ident: ref44/cit44 doi: 10.1074/jbc.M114.557694 – ident: ref70/cit70 doi: 10.1021/cr2001977 – ident: ref18/cit18 doi: 10.1074/jbc.AC119.010611 – ident: ref41/cit41 doi: 10.1093/nar/gky995 – ident: ref8/cit8 doi: 10.1146/annurev-virology-031413-085537 – ident: ref22/cit22 doi: 10.1093/infdis/jix242 – ident: ref7/cit7 doi: 10.1016/j.celrep.2016.05.074 – ident: ref46/cit46 doi: 10.1038/nchembio.405 – ident: ref16/cit16 doi: 10.1002/eji.201847692 – ident: ref56/cit56 doi: 10.1073/pnas.0710389105 – ident: ref57/cit57 doi: 10.1038/nchembio.388 – start-page: 1 year: 2020 ident: ref74/cit74 publication-title: bioRxiv contributor: fullname: Prelli Bozzo C. – ident: ref67/cit67 doi: 10.1021/ja054842f – ident: ref69/cit69 doi: 10.1038/nrm2084 – ident: ref58/cit58 doi: 10.1146/annurev.biochem.052308.105824 – ident: ref37/cit37 doi: 10.1074/jbc.M112.362095 – ident: ref59/cit59 doi: 10.1146/annurev-biochem-060713-035737 – ident: ref45/cit45 doi: 10.1016/j.chembiol.2020.03.004 – volume: 30 start-page: 555 year: 2020 ident: ref29/cit29 publication-title: BioRxiv contributor: fullname: Zang R. – ident: ref43/cit43 doi: 10.1021/acsnano.0c10567 – ident: ref32/cit32 doi: 10.1371/journal.ppat.1004048 – ident: ref65/cit65 doi: 10.1021/acs.jproteome.8b00002 – ident: ref4/cit4 doi: 10.1038/nature10921 – ident: ref51/cit51 doi: 10.1083/jcb.200404100 – ident: ref2/cit2 doi: 10.1016/j.cell.2009.12.017 – ident: ref5/cit5 doi: 10.1128/JVI.00481-13 – ident: ref9/cit9 doi: 10.1016/j.jmb.2013.09.024 – ident: ref68/cit68 doi: 10.1038/nchembio834 – ident: ref12/cit12 doi: 10.1128/JVI.01254-16 – ident: ref62/cit62 doi: 10.1039/D0CC06073A – ident: ref30/cit30 doi: 10.2217/fmb.14.65 – ident: ref6/cit6 doi: 10.1371/journal.ppat.1001258 – ident: ref31/cit31 doi: 10.1021/jacs.6b08733 – ident: ref48/cit48 doi: 10.1074/jbc.M117.800482 – ident: ref10/cit10 doi: 10.1073/pnas.1900784116 – ident: ref47/cit47 doi: 10.1073/pnas.1602244113 – ident: ref63/cit63 doi: 10.1016/j.cbpa.2013.07.031 – ident: ref21/cit21 doi: 10.15252/embr.201642771 – ident: ref39/cit39 doi: 10.1371/journal.pone.0104341 – ident: ref25/cit25 doi: 10.1093/infdis/jiaa224 – ident: ref3/cit3 doi: 10.1038/nature09907 – ident: ref66/cit66 doi: 10.1007/s10858-009-9333-z – ident: ref52/cit52 doi: 10.1083/jcb.200507067 – ident: ref40/cit40 doi: 10.1038/srep24029 – ident: ref49/cit49 doi: 10.1016/j.cell.2010.04.007 – ident: ref54/cit54 doi: 10.1074/jbc.M110.173583 – ident: ref72/cit72 doi: 10.1002/anie.201707554 – ident: ref33/cit33 doi: 10.1038/s41589-018-0213-2 – ident: ref26/cit26 doi: 10.1038/s41586-020-2355-0 – ident: ref35/cit35 doi: 10.1021/acsnano.0c10567 – ident: ref24/cit24 doi: 10.1038/nm.4370 – ident: ref50/cit50 doi: 10.1126/science.1105654 – ident: ref11/cit11 doi: 10.1371/journal.pone.0143539 – ident: ref53/cit53 doi: 10.1073/pnas.1016184107 – ident: ref61/cit61 doi: 10.1021/cr400355w – ident: ref15/cit15 doi: 10.4049/jimmunol.1201305 – ident: ref64/cit64 doi: 10.1016/j.cbpa.2013.08.004 – ident: ref42/cit42 doi: 10.15252/embr.201744100 – ident: ref71/cit71 doi: 10.1002/anie.201914836 – ident: ref36/cit36 doi: 10.1016/j.chom.2013.03.006 – ident: ref20/cit20 doi: 10.26508/lsa.201900542 – ident: ref60/cit60 doi: 10.1038/nchembio0605-13 – ident: ref14/cit14 doi: 10.1038/ni.2525 – ident: ref55/cit55 doi: 10.1038/emboj.2012.15 – ident: ref1/cit1 doi: 10.1016/0092-8674(84)90270-8 – volume: 365 start-page: 176 year: 2019 ident: ref19/cit19 publication-title: Science doi: 10.1126/science.aaw7733 contributor: fullname: Buchrieser J. |
| SSID | ssj0043689 |
| Score | 2.4709654 |
| Snippet | Interferon-induced transmembrane proteins (IFITMs) are
-palmitoylated proteins in vertebrates that restrict a diverse range of viruses.
-palmitoylated IFITM3... Interferon-induced transmembrane proteins (IFITMs) are S -palmitoylated proteins in vertebrates that restrict a diverse range of viruses. S -palmitoylated... |
| SourceID | pubmedcentral crossref pubmed |
| SourceType | Open Access Repository Aggregation Database Index Database |
| StartPage | 844 |
| SubjectTerms | Amino Acid Sequence Antiviral Agents - chemistry Antiviral Agents - pharmacology Binding Sites Cell Membrane - metabolism Cell Membrane - ultrastructure Computational Biology Drug Design Humans Interferons - chemistry Interferons - pharmacology Lipids - chemistry Lipoylation Lysosomes - metabolism Membrane Proteins - metabolism Molecular Dynamics Simulation Protein Binding Protein Conformation RNA-Binding Proteins - metabolism Signal Transduction |
| Title | Site-Specific Lipidation Enhances IFITM3 Membrane Interactions and Antiviral Activity |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33887136 https://pubmed.ncbi.nlm.nih.gov/PMC9112659 |
| Volume | 16 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV07T8MwED5BF1h4P8pLHtjAUNtJHI9VadUOZWkrsUWO7agdSFEf_x9fkhYiwdA51tk6W777cr7vA3gMnYxdEFtqpLPUZ-CaqpYLqIdDupW1VKxS7Hfuj-T7R_zWRZqc538q-Jy9auNhnvtMZ_MXZoqUBS9cKVGnod0Zba5dZFJXBTlqGNDYB_2qQ-ZvE7UotA099WeRv-JM73i3FZ7AUZVPknZ5AE5hz-VncNDZyLidw2Tkk0payMxnM0NQq7qUUSLdfIpbviSD3mA8FGTojfvI5Ujxl7BseFgSnVvSzlFjYoHzmFJt4gImve6406eVlgI1HiGuKNepdpxpizshpMt85OdOmTTy-DiKUi2t0SyTmUZ1N52plGE5VwXCpqHImLiERj7P3TUQDwAllucsEyaQsdHKWG6jwGhvRMeiCU8b3yZfJWVGUpS6OUt-XJVUrmrCVen17VgPmj2KE1ETZG0_tgOQEbv-JZ9NC2ZshQ1RobrZaQW3cMjxoUorpJzdQWO1WLt72F_a9UNxwr4Bo0zRKQ |
| link.rule.ids | 230,315,782,786,887,2770,27934,27935 |
| linkProvider | American Chemical Society |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Site-specific+lipidation+enhances+IFITM3+membrane+interactions+and+antiviral+activity&rft.jtitle=ACS+chemical+biology&rft.au=Garst%2C+Emma+H.&rft.au=Lee%2C+Hwayoung&rft.au=Das%2C+Tandrila&rft.au=Bhattacharya%2C+Shibani&rft.date=2021-05-21&rft.issn=1554-8929&rft.eissn=1554-8937&rft.volume=16&rft.issue=5&rft.spage=844&rft.epage=856&rft_id=info:doi/10.1021%2Facschembio.1c00013&rft_id=info%3Apmid%2F33887136&rft.externalDBID=PMC9112659 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1554-8929&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1554-8929&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1554-8929&client=summon |