Interplay between Intestinal Bacterial Communities and Unicellular Parasites in a Morbidly Obese Population: A Neglected Trinomial

• Published in: Nutrients Vol. 14; no. 15; p. 3211
• Main Authors: Caudet, Jana, Trelis, María, Cifre, Susana, Soriano, José M, Rico, Hortensia, Merino-Torres, Juan F
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05-08-2022; MDPI
• Subjects: Animals; Bacteria; Bacteria - genetics; Bacteroides; Bacteroidetes; Blastocystis; Blastocystis sp; Body mass index; Cardiovascular diseases; Comorbidity; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2; Dientamoeba fragilis; Digestive system; Disease; Epidemics; Escherichia; Eukaryotes; eukaryotic microbiota; Faecalibacterium; Fatty liver; Feces; Feces - microbiology; Firmicutes; Gastrointestinal surgery; Giardia intestinalis; Health risks; Humans; Insulin; Insulin resistance; Lactobacillus; Liver; Liver diseases; Metabolic disorders; metabolic markers; Metabolic syndrome; Metabolism; Metagenomics; Microbiota; Microorganisms; Nutrition research; Obesity; Obesity, Morbid; Parasites; Population; Prevotella; Quality assessment; Questionnaires; Relative abundance; Software; Weight control; Spain; Dientamoeba fragilis; Giardia intestinalis; eukaryotic microbiota; Blastocystis sp; diet; obesity; metabolic markers
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14153211

Obesity is an epidemic causing a metabolic health crisis. Herein, the interactions between the gut prokaryotic and eukaryotic communities, metabolic comorbidities and diet were studied. Stool samples from 56 subjects, 47 with type III obesity and 9 with type II obesity and cardiovascular risk or metabolic disease, were assessed for the richness, diversity and ecology of the bacterial gut community through metagenomics, together with the study of the presence of common unicellular eukaryote parasites (
sp.,
and
) by qPCR. Clinical information regarding metabolic comorbidities and non-alcoholic hepatic fatty liver disease was gathered. To assess the quality of the patients' diet, each participant filled in three dietary questionnaires. The most prevalent parasite
sp. (46.4%), together with
(8.9%), was found to be associated with higher mean diversity indexes regarding non-colonized subjects; the opposite of that which was observed in those with
(16.1%). In terms of phyla relative abundance, with
sp. and
, very slight differences were observed; on the contrary,
was related to an increase in Bacteroidetes and Proteobacteria, and a decrease in Firmicutes and Actinobacteria, presenting the lowest Firmicutes/Bacteroidetes ratio. At genus level,
sp. and/or
was accompanied with an increase in
spp., and a decrease in
spp.,
spp. and
spp., while
was associated with an increase in
spp., and a decrease in
spp.,
spp. and
spp., and the highest
spp./
spp. ratio. Participants with non-alcoholic hepatic fatty liver presented a higher Firmicutes/Bacteroidetes ratio, and those with type 2 diabetes displayed a significantly lower
spp./
spp. ratio, due to an overrepresentation of the genus
spp. The presence of parasites was associated with variations in the richness, diversity and distribution of taxa in bacterial communities, confirming a gain in diversity associated with
sp. and providing different functioning of the microbiota with a potential positive effect on comorbidities such as type 2 diabetes, insulin resistance and metabolic syndrome. Future basic and clinical studies should assess the beneficial or pathogenic effect of these eukaryotes on obese subjects and focus on deciphering whether they may imply a healthier metabolic profile.