Cloning and sequencing of protochlorophyllide reductase

• Published in: Biochemical journal Vol. 265; no. 3; pp. 789 - 798
• Main Authors: Darrah, P M, Kay, S A, Teakle, G R, Griffiths, W T
• Format: Journal Article
• Language: English
• Published: England 01-02-1990
• Subjects: Amino Acid Sequence; Avena sativa; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA - genetics; Edible Grain - genetics; genes; Hydrolysis; Molecular Sequence Data; Nucleic Acid Hybridization; Oxidoreductases - genetics; Oxidoreductases Acting on CH-CH Group Donors; Protein Biosynthesis; Restriction Mapping; RNA - genetics; Transcription, Genetic
• ISSN: 0264-6021; 1470-8728
• DOI: 10.1042/bj2650789

Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase protein.