Effect of Bifidobacterium animalis B/12 administration in healthy dogs

• Published in: Anaerobe Vol. 28; pp. 37 - 43
• Main Authors: Strompfovaa, Viola, Simonovaa, Monika Pogany, Gancarcikovab, Sona, Mudronovab, Dagmar, Farbakovac, Jana, Mad'aric, Aladar, Laukovaa, Andrea
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2014
• Subjects: Alanine Transaminase - blood; Albumins - analysis; Alkaline Phosphatase - blood; Animals; Bifidobacterium; Bifidobacterium - growth & development; Bifidobacterium - metabolism; Bifidobacterium animalis; Blood - immunology; Blood biochemistry; Blood Chemical Analysis; Carboxylic Acids - analysis; Dogs; Feces - chemistry; Feces - microbiology; Female; Haematology; Intestinal microbiota; Leukocytes - immunology; Male; Phagocytosis; Probiotic; Probiotics - administration & dosage; Triglycerides - blood; Probiotic; Bifidobacterium; Haematology; Blood biochemistry; Intestinal microbiota; Dogs
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2014.05.001

Bifidobacterium species constitute the most frequently used health-enhancing bacteria in functional foods or probiotic products, and most of their health benefits have been demonstrated in human or mice studies. However, knowledge of the effects of these bacteria in the canine organism is very limited. In this study, the canine-derived strain Bifidobacterium animalis B/12 (109 CFU) was tested for its effects on faecal microbiota, faecal characteristics, faecal organic acid concentrations, blood biochemistry, haematological and immunological parameters in healthy dogs (C-control, BA-B. animalis B/12 group, 10 dogs in each). The experiment lasted for 49 days with a 14-day treatment period (sample collection at days 0, 7, 14, 21, 28, and 49). A significantly higher population of lactic acid bacteria was detected (day 7) while the counts of coliform bacteria were lower in faeces of the BA group (days 14, 21, 28, 49) compared to control group C. Faecal concentrations of acetic (day 7, 21, 28, 49), acetoacetic (7–49) and valeric acid (14) were higher in contrast to formic acid (day 7–21), which was decreased after the treatment. In blood serum, significantly lower concentrations of triglyceride (day 14) and albumin (day 14, 28, 49) and significantly higher levels of alanine aminotransferase (day 14) and alkaline phosphatase (day 14, 28) were observed in the BA dogs. The phagocytic activity of leukocytes (especially of neutrophils) was higher in dogs after 14-day consumption of B/12 strain (day 14). The results show that many of these effects could also still be recorded several weeks after the treatment period.
•We examine 2-week lasting treatment of Bifidobacterium animalis B/12 in 10 dogs.•Reduction of Gram-negative bacteria in faeces was detected after the treatment.•Faecal levels of acetic, acetoacetic and valeric acid were higher in treated dogs.•Serum triglyceride, albumin was decreased, alanine aminotransferase increased.•Phagocytosis of leukocytes was higher after 14 days of treatment.