Gene Targeting with Viral Vectors

Gene Targeting with Viral Vectors

Genetic manipulation of cells for scientific and therapeutic goals can be achieved by both gene-addition and gene-targeting methods. Gene targeting precisely alters a gene in its natural chromosome location, providing distinct advantages over gene-addition approaches. Classic gene-targeting delivery...

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Bibliographic Details
Published in:Molecular therapy Vol. 12; no. 1; pp. 9 - 17
Main Authors: Hendrie, Paul C, Russell, David W
Format: Journal Article
Language:English
Published: United States Elsevier Limited 01-07-2005
Subjects:
Adenoviridae
Adenoviruses
Dependovirus
Gene Targeting
Gene therapy
Genetic engineering
Genetic Vectors
Plasmids
Retroviridae
Streptococcus infections
Vectors (Biology)
Viruses
United States > US
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Summary:Genetic manipulation of cells for scientific and therapeutic goals can be achieved by both gene-addition and gene-targeting methods. Gene targeting precisely alters a gene in its natural chromosome location, providing distinct advantages over gene-addition approaches. Classic gene-targeting delivery systems (microinjection, electroporation, or calcium phosphate transfection) have led to major scientific advances, but are too inefficient in their current state to be used for some applications, including gene therapy. This review describes the development of gene-targeting vectors based on three types of viruses (retrovirus, adenovirus, and adeno-associated virus) and discusses the design, possible mechanisms of action, and applications of gene-targeting vectors based on adeno-associated virus.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:1525-0016
1525-0024
DOI:10.1016/j.ymthe.2005.04.006