Primary responses of human T cells to mycobacteria: a frequent set of gamma/delta T cells are stimulated by protease-resistant ligands

• Published in: European journal of immunology Vol. 20; no. 5; p. 1175
• Main Authors: Pfeffer, K, Schoel, B, Gulle, H, Kaufmann, S H, Wagner, H
• Format: Journal Article
• Language: English
• Published: Germany 01-05-1990
• Subjects: Antigens, Bacterial - immunology; Chromatography, Gel; Heat-Shock Proteins - immunology; Humans; Lymphocyte Activation; Molecular Weight; Mycobacterium tuberculosis - immunology; Peptide Hydrolases; Receptors, Antigen, T-Cell - physiology; Receptors, Antigen, T-Cell, gamma-delta; T-Lymphocytes - immunology
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830200534

T lymphocyte subsets expressing either T cell receptor alpha/beta or gamma/delta were selected from human peripheral blood T cells and proliferative responses to molecular mass-fractionated mycobacterial lysates were determined. alpha/beta T cells primarily responded to fractions greater than 30 kDa whereas gamma/delta T cells preferentially reacted to fractions less than 3 kDa. Protease digestion abolished the stimulating activities for alpha/beta T cells, confirming that alpha/beta T cells respond to protein components. In contrast, components recognized by gamma/delta T cells proved resistant to protease digestion. In limiting dilution studies, frequencies of proliferating gamma/delta T cells remained virtually unaltered by protease treatment of stimulating lysates, while those of alpha/beta T cells became almost undetectable. Furthermore, only few gamma/delta T cells responded to the 65-kDa heat-shock protein. Our data indicate that, unlike alpha/beta T cells, gamma/delta T cells respond to mycobacterial components which are resistant to vigorous protease digestion.