Examination of the inflammatory response following implantation of titanium plates coated with phospholipids in rats

• Published in: Journal of materials science. Materials in medicine Vol. 22; no. 4; pp. 1015 - 1026
• Main Authors: Kochanowski, Alexandra, Hoene, Andreas, Patrzyk, Maciej, Walschus, Uwe, Finke, Birgit, Luthringer, Bérengère, Feyerabend, Frank, Willumeit, Regine, Lucke, Silke, Schlosser, Michael
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-04-2011; Springer; Springer Nature B.V
• Subjects: Animals; Biocompatible Materials - chemistry; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biomimetics; Cell Proliferation; Ceramics; Chemistry and Materials Science; Composites; Glass; Humans; Immunohistochemistry - methods; Inflammation; Lipids; Macrophages - cytology; Materials Science; Materials Testing; Medical sciences; Natural Materials; Organophosphonates - chemistry; Phosphatidylethanolamines - chemistry; Phospholipids - chemistry; Polymer Sciences; Protective coatings; Rats; Regenerative Medicine - methods; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Time Factors; Titanium; Titanium - chemistry; Transplants & implants; Tissue Macrophage; Pope Coating; Foreign Body Reaction; Contact Angle; Foreign Body Response; Implant; Rat; Rodentia; Phospholipid; Inflammation; Coated material; Plate; Vertebrata; Mammalia; Animal; Surface properties; Titanium; Biomaterial; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-011-4287-6

Implantation of biomaterials like titanium (Ti) causes inflammatory reactions possibly affecting implant functionality. Surface modifications could improve biocompatibility and functionality of implants. Biomembrane-derived phospholipids might be useful as implant coating due to their biomimetic properties. In vitro studies demonstrated beneficial effects for 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamin (POPE) as coating regarding interactions with cells and bacteria. Therefore, this in vivo study aimed at examining local inflammatory reactions after implantation of POPE-coated Ti plates. Ti implants with POPE attached non-covalently or covalent via octadecylphosphonic acid (OPA), with OPA alone and uncoated controls were simultaneously implanted intramuscularly in rats for 7, 14 and 56 days. The peri-implant tissue was quantitatively analyzed by immunohistochemistry for total macrophages, tissue macrophages, T cells, antigen-presenting cells and proliferating cells. Overall, both POPE-coated series were comparable to the controls. Furthermore, no differences were found between POPE coating on a covalently linked OPA monolayer and POPE coating dried from solution. Together with earlier in vitro results, this demonstrates the potential of phospholipids for implant surface modification.