The Fission Yeast Ortholog of the Coregulator SKIP Interacts with the Small Subunit of U2AF

• Published in: Biochemical and biophysical research communications Vol. 284; no. 5; pp. 1148 - 1154
• Main Authors: Ambrozková, Monika, Půta, František, Fuková, Iva, Skružný, Michal, Brábek, Jan, Folk, Petr
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29-06-2001
• Subjects: Cell Cycle; interchromatin granule clusters; Molecular Sequence Data; Nuclear Proteins - metabolism; Ribonucleoproteins - metabolism; Saccharomyces cerevisiae - metabolism; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe; SNW proteins; SNW1/SKIP/NCoA-62 protein; spliceosome; Splicing Factor U2AF; spSNW1 gene; transcription coactivator; Transcriptional Activation; Two-Hybrid System Techniques; U2 auxiliary factor; yeast two-hybrid interaction; interchromatin granule clusters; SNW proteins; yeast two-hybrid interaction; transcription coactivator; Schizosaccharomyces pombe; spliceosome; U2 auxiliary factor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.5108

The mode of action of transcriptional coregulators may involve the recruitment of spliceosome components. Using the two-hybrid screen, we examined the interaction partners of spSNW1, the S. pombe ortholog of the human coregulator SNW1/SKIP/NCoA-62, and found it to interact with the small subunit of the splicing factor U2AF (spU2AF23). The interaction involves the C-terminal parts of spU2AF23 and spSNW1. Tagged variants of both proteins were expressed in S. pombe and the interaction was proved by coprecipitation in nuclear extracts. This interaction would explain the finding of SKIP in nuclear speckles (Mintz, P. J., et al., EMBO J. 18, 4308–4320, 1999) and in reconstituted spliceosomes (Neubauer, G., et al., Nat. Genet. 20, 46–50, 1998). We deleted the spSNW1 gene in the diploid strain and demonstrated that spSNW1 is an essential gene in S. pombe.