A simple, inexpensive, and rapid method to assess antibiotic effectiveness against exoelectrogenic bacteria

• Published in: Biosensors & bioelectronics Vol. 168; p. 112518
• Main Authors: Gao, Yang, Ryu, Jihyun, Liu, Lin, Choi, Seokheun
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-11-2020
• Subjects: Anti-Bacterial Agents - pharmacology; Antimicrobial resistance; Antimicrobial susceptibility testing; Bacteria; Biosensing Techniques; Exoelectrogens; Extracellular electron transfer; Gram-Negative Bacteria; Microbial fuel cell; Microbial Sensitivity Tests; Antimicrobial susceptibility testing; Exoelectrogens; Microbial fuel cell; Antimicrobial resistance; Extracellular electron transfer
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2020.112518

A sufficiently fast and simple antimicrobial susceptibility testing (AST) is urgently required to guide effective antibiotic usages and to surveil the antimicrobial resistance rate. Here, we establish a rapid, quantitative, and high-throughput phenotypic AST by measuring electrons transferred from the interiors of microbial cells to external electrodes. Because the transferred electrons are based on microbial metabolic activities and are inversely proportional to the concentration of potential antibiotics, the changes in electrical outputs can be readily used as a transducing signal to efficiently monitor bacterial growth and antibiotic susceptibility. The sensing is performed by directly measuring the total energy, or all the accumulated microbial electricity, generated by microbial fuel cells (MFCs) arranged in a large-capacity disposable, paper-based testbed. A common Gram-negative pathogenic bacterium Pseudomonas aeruginosa wild-type PAO1 and first-line antibiotic gentamicin (GEN) are used in our experiments. The minimum inhibitory concentration (MIC) values generated from our technique are validated by the gold standard broth microdilution (BMD). Our new approach provides quantitative, actionable MIC results within just 5 h because it measures electricity produced by bacterial metabolism instead of the days needed for growth-observation methods. Moreover, as the equipment needed is simple, common, and inexpensive, our test has immense potential to be adopted in the field or resource-limited hospitals and labs to provide insightful assessments for research and clinical practices.
•Our ingenious device quickly tests antibiotic effectiveness by measuring energy output of bacteria.•The device determines the influence of antibiotics on bacterial growth and metabolic activities.•Our new approach provides quantitative, actionable MIC results within just 5 h.