An investigation of the biochemical properties of tetrazines as potential coating additives

• Published in: Materials Science & Engineering C Vol. 33; no. 4; pp. 1925 - 1934
• Main Authors: Jaiswal, Swarna, Varma, P.C. Rajath, O'Neill, Luke, Duffy, Brendan, McHale, Patrick
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-05-2013
• Subjects: Anti-Bacterial Agents - pharmacology; Antibacterial; Anticorrosion; Antioxidants - metabolism; Bacterial Adhesion - drug effects; Coated Materials, Biocompatible - pharmacology; Coating; Electrochemical Techniques; Fluorescence Recovery After Photobleaching; Gram-Negative Bacteria - cytology; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - growth & development; Gram-Positive Bacteria - cytology; Gram-Positive Bacteria - drug effects; Gram-Positive Bacteria - growth & development; Heterocyclic Compounds, 1-Ring - pharmacology; Kinetics; Magnetic Resonance Spectroscopy; Malondialdehyde - metabolism; Materials Testing; Microbial Sensitivity Tests; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Sol–gel; Spectroscopy, Fourier Transform Infrared; Tetrazine; Anticorrosion; Antibacterial; Coating; Sol–gel; Tetrazine
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2012.12.094

1,2,4,5-Tetrazine and its 3,6-disubstituted derivatives are currently used for a range of industrial and medical applications as they exhibit particular coordination chemistries, characterised by electron and charge transfer phenomena. The aim of the present work is to synthesise two tetrazine derivatives, namely 3,6-dihydrazino-1,2,4,5-tetrazine (DHDTZ) and 1,2,4,5-tetrazine dicarboxylic acid (DCTZ), and determine their antibacterial, antioxidant and anticorrosion characteristics as additives in a sol–gel coating on SS316L steel. The structure of the tetrazines was confirmed by NMR and FTIR while the surface morphology of bacterial cells in their presence was observed by AFM. Their ability to inhibit corrosion on 316L stainless steel was electrochemically determined using a potentiodynamic scanning (PDS) technique. The corrosion inhibition results showed that the acidic DCTZ provided the best corrosion protection. The concentration-dependent antioxidant capacity of the tetrazines was confirmed by both DPPH radical scavenging activity and FRAP assays, showing higher activity for DHDTZ than DCTZ. Furthermore, a DHDTZ doped sol–gel solution was prepared and curing parameter (temperature and time) was optimised for coating on microtitre wells and stainless steel panel. The antibacterial activity of the coated surfaces against Pseudomonas aeruginosa ATCC 27853 and the biofilm forming bacteria Staphylococcus epidermidis CSF 41498 was determined. DHDTZ showed significantly higher antibacterial activities with MIC as low as 31ppm compared to 250ppm for DCTZ.
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► Tetrazine derivatives showed strong antibacterial activity against bacterial pathogens. ► Electrochemical studies confirmed the anticorrosion property of the compounds. ► Significant antioxidant activity was exhibited by the compounds. ► Tetrazine doped sol–gel coating inhibited bacterial growth on 316L stainless steel. ► Tetrazine derivatives can be used as a potential hygiene coating additive.