Effective lactic acid production by two-stage extractive fermentation

• Published in: Journal of fermentation and bioengineering Vol. 79; no. 6; pp. 589 - 593
• Main Authors: Honda, Hiroyuki, Toyama, Yoshio, Takahashi, Hiroshi, Nakazeko, Takuo, Kobayashi, Takeshi
• Format: Journal Article
• Language: English
• Published: Osaka Elsevier B.V 1995; Society for Fermentation and Bioengineering
• Subjects: ACIDE LACTIQUE; ACIDO LACTICO; Alamine 336; ALCOHOLES; ALCOHOLS; ALCOOL; AMINAS; AMINE; AMINES; Biological and medical sciences; Biotechnology; EXTRACCION POR DISOLVENTES; EXTRACTION PAR SOLVANT; FERMENTACION; FERMENTATION; Fundamental and applied biological sciences. Psychology; LACTIC ACID; LACTOBACILLUS; Lactobacillus delbrueckii; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; oleyl alcohol; SOLVENT EXTRACTION; two-stage extractive fermentation; lactic acid; Alamine 336; Lactobacillus delbrueckii; oleyl alcohol; two-stage extractive fermentation; Continuous process; Performance evaluation; Culture medium; Extraction agent; Growth rate; Toxicity; Bacteria; Lactobacillaceae; Lactic acid; Fermentation
• ISSN: 0922-338X
• DOI: 10.1016/0922-338X(95)94753-E

For effective microbial lactic acid production using
Lactobacillus delbrueckii, two-stage extractive fermentation was examined. Extractants were screened from the viewpoints of a high distribution coefficient for lactic acid and less toxicity toward the microorganism. Even if the extractant showed some toxicity toward the microorganism, it was found that a reduction of toxicity was possible by back-extraction using oleyl alcohol. As a result, 40% Alamine 336 diluted with oleyl alcohol, and oleyl alcohol, were selected as the extractant and the back-extractant, respectively. After two-stage extraction by these extractants, the growth rate was improved by the removal of lactic acid. This method was then applied to continuous extractive fermentation using a jar-fermentor. During 4-h extraction, lactic acid accumulation in the broth was significantly suppressed, while the cell growth and glucose consumption rates were also found not to be reduced. After 24 h, the cell concentration attained an OD
660 of 14, which corresponded to a level 1.25 times higher than that of the control culture without extraction. Total lactic acid productivity was 1.4 times level compared with the control culture.