$Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery$
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Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery

[Display omitted] •Use of the frit-inlet channel improves MD-AF4 analysis of LNP-RNA.•A robust method for AF4 separation of LNP-RNA has been developed and tested on two different platform.•The method meets all the ISO standard requirements for AF4 analysis.•The method allowed the analysis of particl...

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Bibliographic Details
Published in:	European journal of pharmaceutics and biopharmaceutics Vol. 163; pp. 252 - 265
Main Authors:	Mildner, R., Hak, S., Parot, J., Hyldbakk, A., Borgos, S.E., Some, D., Johann, C., Caputo, F.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-06-2021
Subjects:	Asymmetric-flow field-flow fractionation Drug Carriers - chemistry Fractionation, Field Flow - methods Frit-inlet channel Humans Lipid-based nanoparticles Lipids - chemistry Nanomedicine Nanomedicine - methods Nanoparticles - chemistry Particle concentration Particle Size Pharmaceutical Preparations - administration & dosage Pharmaceutical Preparations - chemistry Physical–chemical characterisation RNA - administration & dosage RNA - chemistry RNA delivery FF MD-AF4 Particle size FDA NP RT Frit-inlet channel FI PEG2000-DMG CDI EtOH DLS HS NCI-NCL Physical–chemical characterisation BSA LC-MS/MS Particle concentration Rg FBS Rh MC3 Nanomedicine CQAs PBS PSD DSPC Lipid-based nanoparticles TRPS NPhs COV XF AF4 RNA delivery EUNCL UV–Vis PdI MeOH LNP-RNA FWHM LC RI aCSF MALS NTA TEM Asymmetric-flow field-flow fractionation QELS
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