Nuclear glucocorticoid binding in chronic lymphatic leukemia lymphocytes

• Published in: Endocrine research communications Vol. 5; no. 2; p. 91
• Main Authors: Stevens, J, Stevens, Y W, Sloan, E, Rosenthal, R, Rhodes, J
• Format: Journal Article
• Language: English
• Published: United States 1978
• Subjects: Aged; Binding, Competitive; Cell Nucleus - metabolism; Female; Humans; Kinetics; Leukemia, Lymphoid - metabolism; Lymphocytes - metabolism; Male; Middle Aged; Receptors, Glucocorticoid - metabolism; Receptors, Steroid - metabolism; Rosette Formation; Triamcinolone Acetonide - metabolism
• ISSN: 0093-6391
• DOI: 10.3109/07435807809089011

A reliable procedure is described for isolating 3H-triamcinolone acetonide (3H-TA) receptor complexes from purified chronic lymphatic leukemia (CLL) lymphocyte nuclei, based on the use of carbobenzoxy-L-phenylalanine (CBZ-L-phe) to prevent breakdown of hormone-receptor complexes during extraction of nuclei with 0.6M KCl. Using this method, specific nuclear glucocorticoid binding was demonstrated in 14/14 patients with untreated CLL. No correlation was found between levels of nuclear-associated 3H-TA and peripheral white blood cell count or rosetting ability of circulating lymphocytes.