Effect of the mitochondrial antioxidant, Mito Vitamin E, on hypoxic–ischemic striatal injury in neonatal rats: A dose–response and stereological study

• Published in: Experimental neurology Vol. 199; no. 2; pp. 513 - 519
• Main Authors: Covey, Matthew V., Murphy, Michael P., Hobbs, Catherine E., Smith, Robin A.J., Oorschot, Dorothy E.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-06-2006; Elsevier
• Subjects: Animals; Animals, Newborn; Antioxidants - pharmacology; Antioxidants - therapeutic use; Biological and medical sciences; Cavalieri method; Corpus Striatum - drug effects; Corpus Striatum - pathology; Dose-Response Relationship, Drug; Female; Hypoxia-Ischemia, Brain - drug therapy; Hypoxia-Ischemia, Brain - pathology; Injuries of the nervous system and the skull. Diseases due to physical agents; Male; Medical sciences; Medium-spiny neurons; Mito Vitamin E; Neurology; Optical disector method; Perinatal hypoxia–ischemia; Pregnancy; Rats; Rats, Sprague-Dawley; Stereotaxic Techniques; Striatum; Traumas. Diseases due to physical agents; Vascular diseases and vascular malformations of the nervous system; Vitamin E - pharmacology; Vitamin E - therapeutic use; Cavalieri method; Perinatal hypoxia–ischemia; Striatum; Optical disector method; Mito Vitamin E; Medium-spiny neurons; Neonatal; Rat; Cardiovascular disease; E-Vitamins; Mitochondria; Ischemia; Optical method; Human; Oxygen; Rodentia; Perinatal; Perinatal hypoxia-ischemia; Antioxidant; Survival; Vertebrata; Mammalia; Neuron; Newborn; Cell death; Animal; Hypoxia
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/j.expneurol.2005.12.026

A mitochondria-targeted antioxidant, Mito Vitamin E (MitoVit E), has previously been shown to prevent mitochondrial oxidative damage. The aim of this study was to investigate the effect of MitoVit E on neuronal survival in the rat striatum after acute perinatal hypoxia–ischemia. Continuous striatal infusion with 4.35 μM, 43.5 μM, or 148 μM of MitoVit E before, during, and after hypoxia–ischemia was not neuroprotective for striatal medium-spiny neurons. Pre- or posttreatment with 435 μM MitoVit E was neurotoxic. These results suggest that MitoVit E is not significantly neuroprotective for striatal medium-spiny neurons after acute perinatal hypoxic–ischemic brain injury. The results also suggest that mitochondrial oxidative damage does not contribute significantly to the death of striatal medium-spiny neurons after perinatal hypoxia–ischemia.