On-line dialysis of organic acids from a Propionibacterium freudenreichii fermentation: evaluation of a new pH control strategy

• Published in: Applied biochemistry and biotechnology Vol. 76; no. 2; pp. 99 - 105
• Main Authors: Costa, J.P.L.C. da, Schorm, C, Quesada-Chanto, A, Boddeker, K.W, Jonas, R
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-02-1999; Springer Nature B.V
• Subjects: Acids; Bacteria; Biological and medical sciences; Bioreactors; Biotechnology; Dialysis; Fermentation; Fundamental and applied biological sciences. Psychology; Hemodialysis; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Organic acids; Propionibacterium freudenreichii; propionic acid; Studies; Performance evaluation; On line; Propionic acid; Control system; Propionibacterium freudenreichii; Fermentation; Organic acids; Propionibacteriaceae; pH; Bacteria; Actinomycetes; Dialysis; Ion exchange
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:76:2:99

The efficiency of an ion exchange system coupled to a bioreactor to extract on-line inhibitory organic acids produced was evaluated. Batch fermentations without dialysis and fed-batch experiments with and without dialysis of Propionibacterium freudenreichii were conducted. It was possible to keep the propionic acid concentration in the reactor at a low level to avoid complete growth inhibition. Improvements in biomass and propionic acid productivities were achieved when the dialysis system was used. The performance of the dialysis system was improved when a new pH control strategy evaluated in this study was used.