Entamoeba histolytica: Bacterial Expression of a Human Monoclonal Antibody Which Inhibits in Vitro Adherence of Trophozoites

• Published in: Experimental parasitology Vol. 96; no. 1; pp. 52 - 56
• Main Authors: Cheng, Xun-Jia, Ihara, Seiji, Takekoshi, Masataka, Tachibana, Hiroshi
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-09-2000; Elsevier
• Subjects: adherence; Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Base Sequence; Biological and medical sciences; Blotting, Western; Cell Adhesion - immunology; DNA, Protozoan - chemistry; Electrophoresis, Polyacrylamide Gel; Entamoeba histolytica; Entamoeba histolytica - immunology; Erythrocytes - parasitology; Escherichia coli - genetics; Escherichia coli - immunology; Fab fragment; Fundamental and applied biological sciences. Psychology; HRP, horseradish peroxidase; human monoclonal antibody; Humans; IFA, indirect fluorescent-antibody test; lectin; Molecular Sequence Data; PBS, phosphate-buffered saline; Protozoa; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - immunology; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Fab fragment; HRP, horseradish peroxidase; PBS, phosphate-buffered saline; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; adherence; lectin; IFA, indirect fluorescent-antibody test; Entamoeba histolytica; human monoclonal antibody; Human; Protozoa; Host parasite relation; Protozoal disease; Lobosea; Parasite; Parasitosis; Monoclonal antibody; Gene expression; Adhesion; Infection; Trophozoite; Bacteria; Amebiasis; Inhibition; Passive immunization
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1006/expr.2000.4546

Cheng, X.-J., Ihara, S., Takekoshi, M., and Tachibana, H. 2000. Entamoeba histolytica: Bacterial expression of a human monoclonal antibody which inhibits in vitro adherence of trophozoites. Experimental Parasitology96, 52–56.