Delineating the antigenotoxic and anticytotoxic potentials of 4-methylimidazole against ethyl methanesulfonate toxicity in bone marrow cell of swiss albino mice

• Published in: Bratislavské lékarské listy Vol. 117; no. 5; pp. 290 - 294
• Main Authors: Norizadeh Tazehkand, M, Topaktas, M, Yilmaz, M B, Hajipour, O, Valipour, E
• Format: Journal Article
• Language: English
• Published: Slovakia 2016
• Subjects: Animals; Bone Marrow Cells - drug effects; Cell Proliferation - drug effects; Chromosome Aberrations; DNA Damage - drug effects; Ethyl Methanesulfonate - toxicity; Female; Imidazoles - pharmacology; Male; Mice; Mitotic Index; Mutagens - toxicity; antigenotoxicity; ethyl methansulfonate; anticytotoxicity; chromosome aberration; 4-Methylimidazole
• ISSN: 0006-9248
• DOI: 10.4149/BLL_2016_057

4-Methylimidazole (4-MEI) is mostly used in beverages and coloring food, dark beers and common brands of cola drinks, which may contain more than 100 μg of this compound per 12-ounce serving. This study was aimed to investigate the antigenotoxic and anticytotoxic effects of 4-MEI (100, 130 and 160 mg/kg) against ethyl methanesulfonate (240 mg/kg) using chromosome aberrations (CAs) and Mitotic index (MI) tests in bone marrow cells of Swiss Albino Mice at 12 h and 24 h treatment periods. So, the t-test was used for the statistical analysis.In this research, 4-MEI at all concentrations for 12 h treatment period reduced chromosomal aberrations and at 130 and 160 mg/kg concentrations for 24 h treatment period increased chromosomal aberrations induced by EMS (240 mg/kg), but th ese reductions and increases were not significant. Also, intraperitoneal injection of 4-MEI at doses of 100, 130 and 160 mg/kg combined with EMS (240 mg/kg) showed that the mitotic index was decreased at 100 and 130 mg/kg for 12h and 130 mg/kg for 24 h treatment periods, when compared to positive sample (EMS), but did not show any statistically difference from the EMS treated group. It can be concluded that 4-MEI might not be antigenotoxic and protective effects in bone marrow cells of Swiss Albino Mice, because 4-MEI could not reduce the chromosomal aberrations induced by EMS.