Application of dermal microdialysis for the determination of bioavailability of clobetasol propionate applied to the skin of human subjects

• Published in: Skin pharmacology and physiology Vol. 25; no. 1; p. 17
• Main Authors: Au, W L, Skinner, M F, Benfeldt, E, Verbeeck, R K, Kanfer, I
• Format: Journal Article
• Language: English
• Published: Switzerland 01-01-2012
• Subjects: Administration, Cutaneous; Adolescent; Adsorption; Adult; Area Under Curve; Biological Availability; Clobetasol - chemistry; Clobetasol - pharmacokinetics; Female; Glucocorticoids - chemistry; Glucocorticoids - pharmacokinetics; Humans; Lipids; Male; Microdialysis - instrumentation; Microdialysis - methods; Skin - metabolism; Skin - ultrastructure; Skin Absorption; Sodium Chloride; Young Adult
• ISSN: 1660-5535
• DOI: 10.1159/000330489

Dermal microdialysis was used to assess the bioavailability of a topical corticosteroid, clobetasol propionate, following application onto the skin of human subjects. The penetration of clobetasol propionate from a 4% m/v ethanolic solution applied onto 4 sites on one forearm of healthy human volunteers was studied. A lipid emulsion, Intralipid®, was used as the perfusate and linear microdialysis probes with a 2-kDa cutoff were inserted intradermally at the designated sites. The results indicated that Intralipid could be used as a suitable perfusate for in vivo microdialysis of this lipophilic drug of interest. Furthermore, the study clearly demonstrated the application of dermal microdialysis as a valuable tool to assess the bioavailability/bioequivalence of clobetasol propionate penetration into the skin following topical application.