Interactions of calf spleen purine nucleoside phosphorylase with antiviral acyclic nucleoside phosphonate inhibitors: kinetics and emission studies

• Published in: Nucleosides & nucleotides Vol. 18; no. 4-5; p. 875
• Main Authors: Wierzchowski, J, Kulikowska, E, Bzowska, A, Holy, A, Magnowska, L, Shugar, D
• Format: Journal Article
• Language: English
• Published: United States 01-04-1999
• Subjects: Animals; Cattle; Enzyme Inhibitors - pharmacology; Kinetics; Nucleosides - chemistry; Nucleosides - pharmacology; Organophosphorus Compounds - chemistry; Purine-Nucleoside Phosphorylase - antagonists & inhibitors; Purine-Nucleoside Phosphorylase - metabolism; Spectrometry, Fluorescence; Spleen - enzymology
• ISSN: 0732-8311
• DOI: 10.1080/15257779908041588

Association between calf spleen purine nucleoside phosphorylase and a series of phosphonylalkoxyalkyl derivatives of purine bases was studied by inhibition kinetics and fluorimetric titrations. Dissociation constants, determined by fluorimetric titration in phosphate-free conditions, were lower than inhibition constants in 1 mM phosphate, and inhibition was still weaker in 50 mM phosphate, in accord with the postulated bisubstrate analogue character of this class of inhibitors.