Sialic Acid is a Marker of Lung Injury Following Lower Extremities Ischemia/Reperfusion

• Published in: European journal of vascular and endovascular surgery Vol. 27; no. 5; pp. 553 - 558
• Main Authors: Berkan, Ö., Göl, M.K., İçağasioğlu, S., Çetınkaya, Ö., Yildiz, E., Doğan, K., Günay, İ.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-05-2004
• Subjects: Analysis of Variance; Animals; Biomarkers; Enzyme-Linked Immunosorbent Assay; Lung - blood supply; Lung - metabolism; Lung - pathology; Lung injury; Male; N-Acetylneuraminic Acid - physiology; Prospective Studies; Rats; Rats, Sprague-Dawley; Reperfusion Injury - metabolism; Reperfusion Injury - physiopathology; Sialic acid; Statistics, Nonparametric; Torso ischemia and reperfusion; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Torso ischemia and reperfusion; Sialic acid; Tumor necrosis factor-α; Lung injury
• ISSN: 1078-5884; 1532-2165
• DOI: 10.1016/j.ejvs.2004.02.006

Objective. This study tests whether sialic acid is a mediator of the lung injury following lower extremity ischemia/reperfusion (I/R).
Design. Prospective randomised study.
Materials and Methods. Thirty-one Sprague–Dawley rats were randomised into four groups: group 1, aorta was exposed but not clamped; group 2, aorta clamped for 3 h, followed by 1 h of reperfusion; group 3, 50 mg/kg pentoxifylline administrated before the aorta was clamped; and group 4, 1 mg/kg dexametasone administrated before the aorta was clamped. Serial arterial blood samples for blood gas, tumor necrosis factor-α (TNF-α), and total SA (TSA) assay were obtained. The lungs were removed and histologically examined for evidence of injury.
Results. Groups 2, 3, and 4 had significantly higher peak serum TSA concentrations compared with groups 1 (group 1 vs. 2,
p=0.001; group 1 vs. 3,
p=0.002; group 1 vs. 4,
p=0.001). Group 3 had lower peak serum TSA concentration. Groups 2 and 4 had significantly higher peak serum TNF-α concentrations (
p=0.0001) compared with groups 1 and 3. Group 3 had lower peak serum TNF-α concentration. Lower TSA and TNF-α levels are associated with lesser degrees of lung injury.
Conclusions. TSA and TNF appear during events that lead to lung injury following lower extremity I/R.