Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method...

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Published in:	Acta veterinaria (Beograd) Vol. 66; no. 3; pp. 347 - 358
Main Authors:	Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov
Format:	Journal Article
Language:	English
Published:	De Gruyter Open 01-09-2016 Sciendo
Subjects:	nucleotide sequence porcine circovirus 2 porcine parvovirus pseudorabies virus Republic of Montenegro
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Abstract	The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AbstractList	The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
Author	Veljović, Ljubiša Radojičić, Marina Nišavić, Jakov Miković, Radoš Milićević, Vesna Knežević, Aleksandra Krnjaić, Dejan Zorić, Andrea Milić, Nenad Stanojević, Maja
Author_xml	– sequence: 1 givenname: Radoš surname: Miković fullname: Miković, Radoš email: rados.mikovic@gmail.com organization: PhD student, Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia – sequence: 2 givenname: Aleksandra surname: Knežević fullname: Knežević, Aleksandra organization: Virology Department, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia – sequence: 3 givenname: Nenad surname: Milić fullname: Milić, Nenad organization: Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia – sequence: 4 givenname: Dejan surname: Krnjaić fullname: Krnjaić, Dejan organization: Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia – sequence: 5 givenname: Marina surname: Radojičić fullname: Radojičić, Marina organization: Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia – sequence: 6 givenname: Ljubiša surname: Veljović fullname: Veljović, Ljubiša organization: Scientific Veterinary Institute of Serbia, Vojvode Toze 14, Belgrade, Serbia – sequence: 7 givenname: Vesna surname: Milićević fullname: Milićević, Vesna organization: Scientific Veterinary Institute of Serbia, Vojvode Toze 14, Belgrade, Serbia – sequence: 8 givenname: Andrea surname: Zorić fullname: Zorić, Andrea organization: Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia – sequence: 9 givenname: Maja surname: Stanojević fullname: Stanojević, Maja organization: Virology Department, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia – sequence: 10 givenname: Jakov surname: Nišavić fullname: Nišavić, Jakov organization: Department of Microbiology, Faculty of veterinary medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia
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Title	Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro
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