Tissue-specific expression in transgenic rice and Arabidopsis thaliana plants of GUS gene driven by the 5′ regulatory sequences of an anther specific rice gene YY2

• Published in: Plant science (Limerick) Vol. 177; no. 5; pp. 390 - 397
• Main Authors: Kuriakose, Boney, Arun, V., Gnanamanickam, Samuel S., Thomas, George
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01-11-2009; [Ireland]: Elsevier Science Ireland Ltd; Elsevier
• Subjects: Agrobacterium; Agronomy. Soil science and plant productions; Anther; anthers; Arabidopsis thaliana; beta-glucuronidase; Biological and medical sciences; complementary DNA; Fundamental and applied biological sciences. Psychology; gene expression; Genetic engineering applications; Genetics and breeding of economic plants; genome; molecular sequence data; Oryza sativa; Plant breeding: fundamental aspects and methodology; plant development; plant tissues; Promoter; promoter regions; reporter genes; Rice; Tapetum; transcription factors; Transformation; transgenes; transgenic plants; Promoter; Anther; Tapetum; Transformation; Agrobacterium; GUS; GSP; RAGE; ASP; Rice; Monocotyledones; Plant biology; Arabidopsis thaliana; Cruciferae; Gramineae; Dicotyledones; Angiospermae; Bacteria; Regulatory sequence; Transgenic plant; Gram negative bacteria; β-Glucuronidase; Enzyme; Tissue specificity; Glycosylases; Oryza; Glycosidases; Hydrolases; Herbaceous plant; Spermatophyta; Rhizobiaceae; Experimental plant
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2009.06.016

The identification and characterization of promoters,
cis-elements and transcription factors are critical for studying gene expression during growth and development in any organism. Use of specific promoters is an absolute requirement for the expression of foreign genes in plants in a developmentally, spatially and/or temporally regulated manner. The YY2 cDNA has been shown to be expressed specifically in the tapetum cells of rice anther. In this study, a 968
bp upstream regulatory sequence (GenBank accession no.
FJ957881) of the gene was isolated using genome walking method. The isolated fragment was shown to drive anther-specific expression of GUS reporter gene in rice as well as in the dicot plant
Arabidopsis thaliana. Deletion analysis revealed that a ∼400
bp 5′ regulatory region is sufficient to confer anther-specific expression of GUS gene.