Differential Expression of a Putative Copper Homeostasis CutC Gene in Fibroporia radiculosa During Initial Decay of Copper-Treated Wood

• Published in: Forest products journal Vol. 70; no. 4; pp. 469 - 475
• Main Authors: Ohno, Katie M, Bishell, Amy B, Stanosz, Glen R
• Format: Journal Article
• Language: English
• Published: Madison Forest Products Society 22-12-2020
• Subjects: Analysis; Annotations; Bacteria; Chemical properties; Citric acid; Composition; Control; Copper; Copper compounds; Decay; Decay fungi; Enzymes; Fibroporia radiculosa; Forest products; Forest products industry; Fungi; Gene expression; Genes; Genetic aspects; Homeostasis; Laboratories; Physiological aspects; Pine; Proteins; Rot; Testing; Toxicity; Wood; Wood preservatives; Wood products; Wood-decaying fungi; United States
• ISSN: 0015-7473; 2376-9637
• DOI: 10.13073/FPJ-D-20-00043

Abstract Living organisms require copper for several cellular processes. Yet intracellular concentrations of copper must be regulated to avoid toxicity. Not much is known about mechanisms of copper regulation in wood decay fungi. However, one putative annotation for a copper homeostasis CutC gene (FIBRA_00129), found in other brown-rot wood decay fungi, has been annotated in Fibroporia radiculosa . The aim of this study was to evaluate wood mass loss and differential expression of FIBRA_00129 during initial decay of untreated and copper-treated wood by two copper-tolerant F. radiculosa isolates (FP-90848-T and L-9414-SP) compared with copper-sensitive Gloeophyllum trabeum . Untreated southern pine ( Pinus spp.) and ammoniacal copper citrate treated southern pine at three concentrations (0.6%, 1.2%, and 2.4%) were used in a 4-week-long standard decay test. Results showed G. trabeum was unable to decay copper-treated wood while both F. radiculosa isolates successfully decayed southern pine at all copper concentrations. G. trabeum and F. radiculosa L-9414-SP showed no detectable FIBRA_00129 expression over the course of this study. F. radiculosa FP-90848-T showed greater FIBRA_00129 downregulation on copper-treated wood than on untreated wood ( P = 0.003). Additionally, there was greater FIBRA_00129 downregulation in F. radiculosa FP-90848-T at week 3 compared with other weeks ( P = 0.015). Future studies are needed to further evaluate FIBRA_00129 during the decay process to determine its potential role in copper-tolerance.