Cyclin E1 expression and malignancy in meningiomas

• Published in: Clinical neurology and neurosurgery Vol. 190; p. 105647
• Main Authors: Pereira, Benedito Jamilson Araújo, Santana Júnior, Pedro Augustto de, de Almeida, Antonio Nogueira, Cavalcante, Stella Gonçalves, de Melo, Keyde Cristina Martins, de Aguiar, Paulo Henrique Pires, Paiva, Wellingson da Silva, Oba-Shinjo, Sueli Mieko, Marie, Suely Kazue Nagahashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-03-2020; Elsevier Limited
• Subjects: Brain cancer; Cell cycle; Cyclin E1; Cyclin-dependent kinases; E1 protein; Gene expression; Kinases; Malignancy; Medical prognosis; Meningioma; Neurology; Patients; Proteins; Statistical analysis; Surgery; Tumors; United States > US; Sweden; Germany; Cyclin E1; Malignancy; Meningioma
• ISSN: 0303-8467; 1872-6968
• DOI: 10.1016/j.clineuro.2019.105647

•Cyclin E1 overexpression was associated with meningiomas of higher malignancy.•Nuclei positivity was observed among the fibrous subtype of grade I meningiomas.•In the present study, CCNE1 expression level reliably predicted malignant grade II meningiomas.
The aim of the present study was to analyze if the pathway Skp2-p27-cyclin E1 could also be a tumor progression marker for meningiomas.
We used quantitative real-time PCR to assess the relative expression levels of the genes coding for cyclin E1 (CCNE1), Skp2 (SKP2), and p27 (P27). The expression levels were compared in grades I to III meningiomas and among different histological subtypes of grade I meningiomas.
Anaplastic meningiomas accounted for 4.9%, atypical meningiomas for 23.5% and grade I meningiomas for 71.6%.CCNE1 expression level was significantly higher in grade II compared to grade I meningiomas (p = 0.0027), and its expression level reliably predicts grade II meningiomas (ROC AUC = 0.731, p = 0.003). CCNE1 expression also correlated with SKP2 and P27 expression levels in grade I meningiomas (r = 0.539, p < 0.0001 and r = 0.687, p = <0.0001, respectively for CCNE1/SKP2 and CCNE1/P27, Spearman’s test). Fibrous subtype among grade I meningiomas presented the highest expression levels of CCNE1, SKP2 and P27. Higher expression of cyclin E1 protein was detected in the nuclei of atypical meningiomas compared to grade I meningiomas.
CCNE1 expression level predicts meningioma malignancy, and the fibrous subtype presents the highest gene expression levels among grade I meningiomas.