Paraprevotella clara gen. nov., sp. nov. and Paraprevotella xylaniphila sp. nov., members of the family 'Prevotellaceae' isolated from human faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 8; pp. 1895 - 1900
• Main Authors: Morotomi, Masami, Nagai, Fumiko, Sakon, Hiroshi, Tanaka, Ryuichiro
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-08-2009; Society for General Microbiology
• Subjects: Acetic Acid - metabolism; Anaerobiosis; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Catalase - metabolism; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Molecular Sequence Data; Phylogeny; Prevotella - classification; Prevotella - genetics; Prevotella - isolation & purification; Prevotella - physiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Succinic Acid - metabolism; Systematics; Human; New genus; Description; Taxonomy; Bacteria; New species; Feces
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.008169-0

Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan
Correspondence Masami Morotomi masami-morotomi{at}yakult.co.jp
Two anaerobic, non-spore-forming, pleomorphic, Gram-negative rods, designated YIT 11840 T and YIT 11841 T , were isolated from human faeces. The organisms were catalase-negative, produced succinic and acetic acids as end products of glucose metabolism and had DNA G+C contents of approximately 48–49 mol%. Although the phenotypic characteristics of these two strains were very similar, analysis of their 16S rRNA gene sequences showed that they are only distantly related (93.8 %), indicating that they represent two different species. A comparative sequence analysis revealed that these two species are members of the family ‘ Prevotellaceae ’ but are phylogenetically distant (<88 % sequence similarity) from the known genera belonging to this family, including Prevotella , Hallela and Xylanibacter . On the basis of the phylogenetic analysis and physiological tests, strains YIT 11840 T and YIT 11841 T represent two novel species of a new genus, for which the names Paraprevotella clara gen. nov., sp. nov. (type strain YIT 11840 T =JCM 14859 T =DSM 19731 T ), the type species, and Paraprevotella xylaniphila sp. nov. (type strain YIT 11841 T =JCM 14860 T =DSM 19681 T ) are proposed.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains YIT 11840 T and YIT 11841 T are AB331896 and AB331897 .