Evaluation of laser phototherapy (λ 780 nm) after dental replantation in rats

• Published in: Dental traumatology Vol. 32; no. 6; pp. 488 - 494
• Main Authors: de Carvalho, Fabíola Bastos, Andrade, Aline Silva, Barbosa, Artur Felipe dos Santos, Aguiar, Márcio Cajazeira, Cangussu, Maria Cristina Teixeira, Pinheiro, Antônio Luiz Barbosa, Ramalho, Luciana Maria Pedreira
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01-12-2016
• Subjects: Animals; Dentistry; laser therapy; laser therapy, low‐Level; low-Level; Low-Level Light Therapy; Rats; Rats, Wistar; Root Resorption; tooth injuries; Tooth Replantation; Tooth Root; laser therapy, low-Level; tooth injuries; root resorption
• ISSN: 1600-4469; 1600-9657
• DOI: 10.1111/edt.12289

Background/Aim
Tooth replantation is the treatment of choice in cases of avulsion although the outcomes are variable. The teeth can be lost due to external root resorption. The aim of this study was to histologically assess of the effect of laser phototherapy (λ780 nm) on replanted teeth in rats.
Material and Methods
Sixty Wistar Albinus rats had their maxillary right incisors extracted and were then divided into four groups: G1–absence of storage medium; G2–milk as storage medium; G3–milk as storage medium followed by a laser irradiation of the root surfaces and entrance of the alveolus (λ = 780 nm; P = 70 mW; CW; DE = 21 J/cm2); G4–milk as storage medium, laser irradiation as in G3 before replantation. After this procedure, laser irradiation was performed on the buccal and palatal mucosa (8.4 J/cm2 per session) every 48 h for 15 days. The animals were euthanized 15, 30, and 60 days after replantation.
Results
The histological results showed that after 15 days, G4 exhibited intense chronic inflammation with the presence of clastic cells and moderate external inflammatory root resorption (P < 0.05) when compared with G3, in which these outcomes were not observed. At the 30th day, G1, G2, and G4 showed chronic inflammation varying from discrete to moderate, as well as intense external inflammatory root resorption. G3 remained without any inflammation and external inflammatory root resorption up to the 60th day.
Conclusions
The use of laser phototherapy on the root surface and at the entrance of the alveolus prior to replantation had a positive biomodulative effect on alveolar repair after tooth replantation in rats.