Clinical relevance of preformed IgG and IgM antibodies against donor endothelial progenitor cells in recipients of living donor kidney grafts

• Published in: Clinical transplantation Vol. 30; no. 2; pp. 124 - 130
• Main Authors: Daniel, Volker, Sadeghi, Mahmoud, Suesal, Caner, Scherer, Sabine, Tran, Hien, Gombos, Petra, Trojan, Karina, Morath, Christian, Opelz, Gerhard
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01-02-2016
• Subjects: Adult; antibodies against endothelial progenitor cells; Endothelial Progenitor Cells - immunology; Female; Follow-Up Studies; Glomerular Filtration Rate; graft dysfunction; Graft Rejection - diagnosis; Graft Rejection - epidemiology; Graft Rejection - etiology; Graft Survival; Histocompatibility Testing; Humans; Immunoglobulin G - blood; Immunoglobulin M - blood; Isoantibodies - blood; Isoantibodies - immunology; Kidney Failure, Chronic - surgery; Kidney Function Tests; Kidney Transplantation; living donor kidney grafts; Living Donors; Male; Middle Aged; Postoperative Complications; preformed EPC antibodies; Prognosis; rejection; Risk Factors; Transplant Recipients; XM-ONE; preformed EPC antibodies; graft dysfunction; XM-ONE; living donor kidney grafts; antibodies against endothelial progenitor cells; rejection
• ISSN: 0902-0063; 1399-0012
• DOI: 10.1111/ctr.12665

Background
Literature reports suggest that non‐HLA‐antibodies against human endothelial progenitor cells (EPC) can be detected in pre‐transplant recipient serum and that EPC antibodies can have a deleterious influence on the graft.
Methods
We investigated 71 renal transplant recipients from living donors for a possible influence of pre‐transplant donor‐specific IgG and/or IgM recipient antibodies against EPC of the donor using the flow cytometric XM‐ONE cross‐match.
Results
Eight of the 71 patients developed acute biopsy‐proven rejection. Two of these patients showed IgM antibodies against EPC prior to transplantation while the other six patients had neither IgG nor IgM EPC antibodies. Conversely, pre‐transplant IgG or IgM antibodies against EPC were detected in 19 patients without acute rejection (3 × both IgG and IgM, 1 × IgG and 15 × IgM). The remaining 44 patients had neither EPC antibodies nor experienced rejection. Comparing serum creatinine levels at one month and one yr post‐transplant within and among the three patient groups revealed that serum creatinine levels were similar in patients with or without EPC antibodies (p > 0.05).
Conclusion
In this series of 71 recipients with living donor kidneys, pre‐transplant EPC antibodies detected with the XM‐ONE test kit were neither associated with acute rejection nor with graft function at one month or one yr.