Construction and characterization of recombinant human C9 or C7 linked to single Chain Fv directed to CD25

•Cancer cells resist cytotoxicity by the Complement membrane attack complex, MAC.•Complement resistance reduces potency of therapeutic antibodies.•Anti-CD25 humanized single chain Fv was fused to Complement protein C9, ScFv-C9.•Recombinant scFv-C9 increases MAC deposition on and death of CD25-positi...

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Bibliographic Details
Published in:	Molecular immunology Vol. 55; no. 3-4; pp. 400 - 408
Main Authors:	Raitses Gurevich, Maria, Fishelson, Zvi
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-10-2013
Subjects:	CD25 Cell Line, Tumor Complement Complement C7 - chemistry Complement C7 - genetics Complement C7 - metabolism Complement C9 - chemistry Complement C9 - genetics Complement C9 - metabolism Complement Membrane Attack Complex - chemistry Complement Membrane Attack Complex - genetics Complement Membrane Attack Complex - metabolism Cytotoxicity, Immunologic HEK293 Cells Humans Immunoconjugates - chemistry Immunoconjugates - genetics Immunotherapy Interleukin-2 Receptor alpha Subunit - metabolism Neoplasms - immunology Neoplasms - pathology Neoplasms - therapy Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism scFv Single-Chain Antibodies - chemistry Single-Chain Antibodies - genetics Single-Chain Antibodies - metabolism HIS C7 C5b-9 scFv C9 NHS Complement CD25 MAC
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