Effect of selection on genetic variability in yellow passion fruit

The objective of this study was to evaluate the genetic diversity in improved (IG) and unimproved germplasm (UIG) of yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg), based on ISSR (Inter Simple Sequence Repeat) markers. The genotypes were grouped by neighbor joining clustering and pr...

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Published in:Crop breeding and applied biotechnology Vol. 12; no. 4; pp. 253 - 260
Main Authors: Costa, J.L, Jesus, O.N. de, Oliveira, G.A.F, Oliveira, E.J. de
Format: Journal Article
Language:English
Published: Vicosa Crop Breeding and Applied Biotechnology 01-12-2012
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Summary:The objective of this study was to evaluate the genetic diversity in improved (IG) and unimproved germplasm (UIG) of yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg), based on ISSR (Inter Simple Sequence Repeat) markers. The genotypes were grouped by neighbor joining clustering and principal component analysis. Regardless of the genotype, a high number of polymorphic bands was observed, aside from several specific fragments of the groups, according to the level of improvement. Unimproved genotypes had a higher number of polymorphic fragments. The hierarchical and principal component clustering coincided in the formation of two rather distinct groups (IG and UIG). The molecular analysis of variance to check the differentiation between groups showed 57 and 43% of the variation within and between groups, respectively. This study demonstrated the potential of ISSR to determine molecular polymorphism in yellow passion fruit and that breeding has narrowed the genetic variability. Objetivou-se avaliar a diversidade genética em germoplasma melhorado (IG) e não melhorado (G-NM) de maracujazeiro amarelo (Passiflora edulis Sims f. flavicarpa Deg), com o uso de marcadores do tipo ISSR (Inter Simple Sequence Repeats). Os genótipos foram agrupados pelo método de neighbor joining e por análise de componentes principais. Foi observado alto número de bandas polimórficas, independente do tipo de genótipo, além de diversos fragmentos específicos dos grupos, de acordo com o nível de melhoramento. Genótipos não melhorados apresentaram maior número de fragmentos polimórficos. O agrupamento hierárquico e de componentes principais foram concordantes na formação de dois grupos bem distintos (IG e G-NM). A análise de variância molecular, utilizada para verificar a diferenciação entre os grupos, evidenciou que 57 e 43% da variação estão entre e dentro dos grupos, respectivamente. Este estudo evidenciou o potencial de uso dos ISSR na determinação do polimorfismo molecular em maracujazeiro amarelo e que o melhoramento tem reduzido a variabilidade genética.
Bibliography:F30
2013001491
ISSN:1518-7853
1984-7033
1984-7033
DOI:10.1590/S1984-70332012000400004