A liquid culture system for Agrobacterium-mediated transformation of tomato ( Lycopersicon esculentum L. Mill.)

• Published in: Plant science (Limerick) Vol. 168; no. 1; pp. 121 - 130
• Main Authors: Velcheva, Margarita, Faltin, Zehava, Flaishman, Moshe, Eshdat, Yuval, Perl, Avihai
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 2005; Elsevier Science
• Subjects: Agrobacterium; Agrobacterium transformation; Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; Biological and medical sciences; cultivars; culture media; Fundamental and applied biological sciences. Psychology; Genetic engineering applications; genetic transformation; genetic variation; genetic vectors; Genetics and breeding of economic plants; in vitro regeneration; Liquid selection; Lycopersicon esculentum; micropropagation; molecular sequence data; nucleotide sequences; Plant breeding: fundamental aspects and methodology; Regeneration; Solanum lycopersicum var. lycopersicum; Tomato; tomatoes; transgenic plants; MSI; B5EL; GUS; Liquid selection; MS; IAA; TDZ; Tomato; Regeneration; B5; cv; Agrobacterium transformation; MSE; PCR; BA; Dicotyledones; Angiospermae; Lycopersicon esculentum; Explant; Above ground plant part; Spermatophyta; Solanaceae
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2004.07.037

Two systems for
Agrobacterium transformation and subsequent plant regeneration, on solidified media and in liquid culture, were developed and employed on different commercial tomato cultivars. The efficiencies of transformation and regeneration were studied. After selection on solid media following transformation, approximately one-quarter of the regenerated plants were non-transformed regenerants (escapes). On the other hand, an improved and highly efficient system for regeneration of transformed explants was developed while using liquid cultures. This is due not only to the better distribution of the selective agent in the liquid medium, but also to differences in the origin of shoot initiation between the solidified and the liquid media. Optimal
Agrobacterium-mediated transformation, shoot regeneration and selection of transformed tomato plants were demonstrated in the present study by using, for the first time, liquid cultures.