5-Methylcytosine containing CG decamer as Z-DNA embedded sequence for a potential Z-DNA binding protein probe

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 37; no. 9; pp. 485 - 497
• Main Authors: Vongsutilers, Vorasit, Sawaspaiboontawee, Kulwadee, Tuesuwan, Bodin, Shinohara, Yoko, Kawai, Gota
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01-01-2018; Taylor & Francis Ltd
• Subjects: 5-methylcytosine; Biosensors; circular dichroism; Deoxyribonucleic acid; DNA; DNA structure; Handedness; NMR; Nucleotide sequence; Oligonucleotides; Z-DNA; Z-DNA binding protein; 5-methylcytosine; NMR; circular dichroism; Z-DNA; Z-DNA binding protein
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1080/15257770.2018.1498512

Attempting to elucidate biological significance of the left-handed Z-DNA is a research challenge due to Z-DNA potential role in many diseases. Discovery of Z-DNA binding proteins has ignited the interest in search for Z-DNA functions. Biosensor with Z-DNA forming probe can be useful to study the interaction between Z-DNA conformation and Z-DNA binding proteins. In this study, 5-methylcytosine (
m
C) containing CG decamers were characterized for their suitability to form Z-DNA and to be used in Z-DNA forming probe. The 5′-thiol oligonucleotide embedded with 5′-
m
CG
m
CG
m
CG
m
CG
m
CG-3′ was designed and developed as a potential Z-DNA forming probe for Z-DNA binding protein screening.