Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity

• Published in: Protoplasma Vol. 233; no. 3-4; pp. 203 - 207
• Main Authors: Souguir, D, Ferjani, E, Ledoigt, G, Goupil, P
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01-11-2008; Springer Vienna; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cell Biology; Chromosomal aberrations; Chromosomes, Plant - drug effects; Copper - pharmacology; DNA Damage; Dose-Response Relationship, Drug; Environmental Pollutants - pharmacology; genotoxicity; Heavy metal; Legumes; Life Sciences; Meristem - drug effects; Microbiology; Micronuclei; Micronucleus Tests; Original Article; Pisum sativum - drug effects; Pisum sativum - genetics; Plant Roots - drug effects; Plant Sciences; root tips; Vicia faba - drug effects; Vicia faba - genetics; Zoology; Root tips; Chromosomal aberrations; Micronuclei; Genotoxicity; Heavy metal
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-008-0004-9

The potential genotoxicity of Cu²⁺ was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu²⁺ caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal on V. faba root meristems. Cu²⁺ induced chromosomal alterations at the lowest concentration used (2.5 mM) when incubated for 42 h, indicating the potent mutagenic effect of this ion. A spectrum of chromosomal abnormalities was observed in V. faba root meristems, illustrating the genotoxic events leading to micronuclei formation.