A clinical assessment of direct electrochemical urate measurements

• Published in: Talanta (Oxford) Vol. 68; no. 5; pp. 1463 - 1468
• Main Authors: Dutt, Jodi S.N., Livingstone, Callum, Cardosi, Marco F., Wilkins, Shelley J., Davis, James
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 28-02-2006; Oxford Elsevier
• Subjects: Analytical chemistry; Biomedical; Carbon fibre; Chemistry; Composites; Diagnosis; Electroanalysis; Electrochemical; Electrochemical methods; Electrode; Exact sciences and technology; General, instrumentation; Hypertension; Purines; Spectrometric and optical methods; Urate; Hypertension; Purines; Electroanalysis; Composites; Electrode; Urate; Carbon fibre; Diagnosis; Biomedical; Electrochemical; Performance evaluation; Chemical analysis; Laminate; Electrochemical method; Colorimetry; Serum; Peroxidase; Carbon fiber; Disposable; Biochemical analysis; Enzyme; Chemical sensor; Carbon; Peroxidases; Oxidoreductases; Comparative study
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2005.08.020

The response characteristics of various carbon substrates towards the direct oxidative measurement of urate and other key purine biomarkers have been compared. A novel carbon fibre laminate assembly has been proposed as an alternative substrate for the preparation of disposable sensing strips. The fabrication method is generic and readily transferable to a number of sensor applications. Its performance in the determination of urate within biofluids (serum and plasma) has been critically assessed. An inter-laboratory pilot study demonstrated the bioanalytical efficacy of the approach with the responses validated through comparison with the standard colorimetric (uricase/peroxidase) assay.