Ultrastructural and immunohistochemical investigation on the gills of the teleost, Thalassoma pavo L., exposed to cadmium

• Published in: Acta histochemica Vol. 113; no. 2; pp. 201 - 213
• Main Authors: Brunelli, Elvira, Mauceri, Angela, Maisano, Maria, Bernabò, Ilaria, Giannetto, Alessia, De Domenico, Elena, Corapi, Barbara, Tripepi, Sandro, Fasulo, Salvatore
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-02-2011
• Subjects: Animals; Apoptosis - drug effects; Biomarkers - analysis; Cadmium; Cadmium - analysis; Cadmium - pharmacology; Calmodulin - analysis; Caspase 3 - analysis; Caspase 3 - metabolism; Dose-Response Relationship, Drug; Electron microscopy; Epithelium - chemistry; Epithelium - drug effects; Epithelium - ultrastructure; Gills; Gills - chemistry; Gills - drug effects; Gills - ultrastructure; Immunohistochemistry; Metallothionein - analysis; Neuropeptides - analysis; Perciformes - anatomy & histology; Perciformes - metabolism; Proliferating Cell Nuclear Antigen - analysis; Teleost; Teleostei; Thalassoma pavo; Ultrastructure; Immunohistochemistry; Teleost; Cadmium; Thalassoma pavo; Ultrastructure; Gills; Electron microscopy
• ISSN: 0065-1281; 1618-0372
• DOI: 10.1016/j.acthis.2009.10.002

An investigation was conducted to determine the effects of the heavy metal, cadmium (Cd), on the gills of the teleost fish,
Thalassoma pavo Linnaeus, 1758. The fishes were exposed to several sublethal concentrations of cadmium (10, 40, 60 and 120
μM (mg/L)) for a period of 48, 96 and 192
h. The value of the LC50 after 96
h of cadmium exposure, determined using the System of Finney, was equal to 128.3
μM. The gills of the fishes were examined by light and electron microscopy. Toxic, apoptotic and cadmium effects were analyzed using some neuropeptides, metallothioneins (MT), caspase 3, PCNA and calmodulin, as bioindicators, respectively. The results showed that the alterations in the gills were proportional to the exposure periods and concentrations of the metal, which were found to be both dose and time dependent. The biological responses in the gills of the tested animals are discussed in relation to results obtained by analysis of the biomarkers. These data may be used for the planning of a model to determine biological risk in the marine environment and may be particularly useful to investigate organisms exposed to cadmium.