Prenatal ozone exposure programs a sexually dimorphic susceptibility to high‐fat diet in adolescent Long Evans rats

• Published in: The FASEB journal Vol. 36; no. 12; pp. e22664 - n/a
• Main Authors: Stewart, Erica J., Dye, Janice A., Schladweiler, Mette C., Phillips, Pamela M., McDaniel, Katherine L., Richards, Judy H., Grindstaff, Rachel D., Padgett, William T., Moore, Makala L., Hill, Donna, Gordon, Christopher J., Kodavanti, Urmila P., Miller, Colette N.
• Format: Journal Article
• Language: English
• Published: United States 01-12-2022
• Subjects: Adolescent; air pollution; Animals; Diet, High-Fat - adverse effects; DOHaD; Female; Fetal Growth Retardation; fetal programming; high fat diet; Humans; Male; obesity; Obesity - metabolism; ozone; Ozone - toxicity; Pregnancy; Rats; Rats, Long-Evans; sex differences; Vitamins; DOHaD; air pollution; fetal programming; high fat diet; obesity; ozone; sex differences
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.202201514R

Altered fetal growth, which can occur due to environmental stressors during pregnancy, may program a susceptibility to metabolic disease. Gestational exposure to the air pollutant ozone is associated with fetal growth restriction in humans and rodents. However, the impact of this early life ozone exposure on offspring metabolic risk has not yet been investigated. In this study, fetal growth restriction was induced by maternal inhalation of 0.8 ppm ozone on gestation days 5 and 6 (4 hr/day) in Long Evans rats. To uncover any metabolic inflexibility, or an impaired ability to respond to a high‐fat diet (HFD), a subset of peri‐adolescent male and female offspring from filtered air or ozone exposed dams were fed HFD (45% kcal from fat) for 3 days. By 6 weeks of age, male and female offspring from ozone‐exposed dams were heavier than offspring from air controls. Furthermore, offspring from ozone‐exposed dams had greater daily caloric consumption and reduced metabolic rate when fed HFD. In addition to energy imbalance, HFD‐fed male offspring from ozone‐exposed dams had dyslipidemia and increased adiposity, which was not evident in females. HFD consumption in males resulted in the activation of the protective 5'AMP‐activated protein kinase (AMPKα) and sirtuin 1 (SIRT1) pathways in the liver, regardless of maternal exposure. Unlike males, ozone‐exposed female offspring failed to activate these pathways, retaining hepatic triglycerides following HFD consumption that resulted in increased inflammatory gene expression and reduced insulin signaling genes. Taken together, maternal ozone exposure in early pregnancy programs impaired metabolic flexibility in offspring, which may increase susceptibility to obesity in males and hepatic dysfunction in females.