Genomic DNA “finger-printing” in diagnostic virology to clarify discrepant HIV results

Highlights • AmpF l STR® Identifiler® PCR Amplification Kit can clarify sample contamination. • Acellular serum would not significantly contribute contaminating human genomic DNA. • Perform human gDNA analysis before starting public health investigation. • Clinical Virologists should have access to...

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Bibliographic Details
Published in:	Journal of clinical virology Vol. 73; pp. 36 - 41
Main Authors:	Sudhanva, Malur, Senior-McKenzie, Tania, Hembrom, Rajesh, Clark, Barnaby
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2015
Subjects:	Adult Allergy and Immunology AmpFlSTR Diagnostic Errors Discrepant HIV DNA - analysis DNA Fingerprinting - methods Female Fingerprinting Forensic Genomic DNA HIV - immunology HIV Antibodies - blood HIV Infections - diagnosis HIV Infections - immunology Human immunodeficiency virus Humans Infectious Disease Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards Pregnancy Pregnant Prenatal Diagnosis Sensitivity and Specificity Pregnant AmpF lSTR ribonucleic acid RNA United Kingdom Accreditation Service Forensic UKAS HIV Fingerprinting genomic deoxyribonucleic acid Discrepant HIV Genomic DNA human immunodeficiency virus gDNA AmpFlSTR
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Summary:	Highlights • AmpF l STR® Identifiler® PCR Amplification Kit can clarify sample contamination. • Acellular serum would not significantly contribute contaminating human genomic DNA. • Perform human gDNA analysis before starting public health investigation. • Clinical Virologists should have access to genomic DNA “finger-printing” service.
Bibliography:	ObjectType-Case Study-2 SourceType-Scholarly Journals-1 ObjectType-Feature-4 content type line 23 ObjectType-Report-1 ObjectType-Article-3 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2015.10.013