Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study
A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC...
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| Published in: | Journal of chemistry Vol. 2013; no. 2013; pp. 1 - 7 |
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| Format: | Journal Article |
| Language: | English |
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Cairo, Egypt
Hindawi Puplishing Corporation
01-01-2013
Hindawi Publishing Corporation Hindawi Limited |
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| Abstract | A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC system. The separation was realized on an analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interassay coefficients of variation were below 10%. The recoveries were as follows: 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for α-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for β-carotene. The levels of selected fat-soluble vitamins in plasma of patients with cardiovascular disease were measured and discussed. |
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| AbstractList | A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC system. The separation was realized on an analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interassay coefficients of variation were below 10%. The recoveries were as follows: 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for α-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for β-carotene. The levels of selected fat-soluble vitamins in plasma of patients with cardiovascular disease were measured and discussed. |
| Author | Drábková, Petra Kand’ár, Roman Novotná, Pavla |
| Author_xml | – sequence: 1 fullname: Kand’ár, Roman – sequence: 2 fullname: Novotná, Pavla – sequence: 3 fullname: Drábková, Petra |
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| CitedBy_id | crossref_primary_10_1016_j_foodchem_2015_10_077 crossref_primary_10_3390_nu13072280 crossref_primary_10_1002_jssc_201700492 crossref_primary_10_1017_S0007114522001398 crossref_primary_10_1038_s41430_018_0236_5 crossref_primary_10_1007_s00394_023_03314_6 crossref_primary_10_5897_AJFS2022_2170 crossref_primary_10_24857_rgsa_v18n9_148 crossref_primary_10_53393_rial_2016_v75_33509 crossref_primary_10_1007_s12161_017_0799_0 crossref_primary_10_1080_09291016_2017_1333199 crossref_primary_10_1186_s12885_020_06839_9 crossref_primary_10_17116_labs2016518_18 |
| Cites_doi | 10.1097/HJR.0b013e328325d807 10.1016/S0009-8981(97)00224-6 10.1093/carcin/bgn072 10.1117/1.2398884 10.1021/jp1100889 10.1373/clinchem.2005.056374 10.1002/(SICI)1099-0801(199803/04)12:2<89::AID-BMC732>3.0.CO;2-0 10.1016/j.jchromb.2009.02.051 10.1007/s00216-003-2288-0 10.1093/clinchem/33.9.1585 10.1016/j.jchromb.2006.04.004 10.1093/ajcn/88.3.747 10.1016/0076-6879(92)13120-M 10.1016/S0009-3084(02)00164-0 10.2310/6650.2007.06043 10.1079/BJN2000248 10.5858/arpa.2011-0426-LE 10.3109/10715762.2011.564168 10.1017/S0007114507868486 10.1016/j.jchromb.2003.12.025 10.1006/abio.1995.0009 10.1093/clinchem/40.3.411 10.1152/physrev.00047.2003 10.1016/j.chroma.2009.01.101 10.1002/biof.5520070106 10.1016/j.ab.2008.06.038 10.1016/S0955-2863(01)00207-8 10.1016/S0009-8981(98)00103-X |
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| Copyright | Copyright © 2013 Roman Kand’ár et al. |
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L. – ident: e_1_2_6_21_2 doi: 10.1006/abio.1995.0009 – ident: e_1_2_6_20_2 doi: 10.1093/clinchem/40.3.411 – ident: e_1_2_6_6_2 doi: 10.1152/physrev.00047.2003 – ident: e_1_2_6_2_2 doi: 10.1016/j.chroma.2009.01.101 – ident: e_1_2_6_11_2 doi: 10.1002/biof.5520070106 – ident: e_1_2_6_14_2 doi: 10.1016/j.ab.2008.06.038 – ident: e_1_2_6_3_2 doi: 10.1016/S0955-2863(01)00207-8 – ident: e_1_2_6_18_2 doi: 10.1016/S0009-8981(98)00103-X |
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| Snippet | A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various... A method is described here for the simultaneous determination of retinol, α ‐tocopherol, lycopene, and β ‐carotene in human plasma. The effectiveness of... |
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| Title | Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study |
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