Hypersensitive and selective biosensing based on microfiber interferometry and molecular imprinted nanoparticles

• Published in: Biosensors & bioelectronics Vol. 141; p. 111347
• Main Authors: Shrivastav, Anand M., Sharma, Gaurav, Jha, Rajan
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-09-2019
• Subjects: Biosensing Techniques - instrumentation; Cascaded taper fiber; Equipment Design; Interferometry - instrumentation; Limit of Detection; Methyl Parathion - analysis; Molecular Imprinting; Nanoparticles - chemistry; Optical sensor; Parathion methyl; Pesticide detection; Pesticides - analysis; Water - analysis; Water Pollutants, Chemical - analysis; Parathion methyl; Optical sensor; Cascaded taper fiber; Pesticide detection; Molecular imprinting
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.111347

The molecular imprinting techniques with interferometric platform are promising for next-generation optical sensors for online and remote biosensing and device applications. This technique has shown a tremendous potential to provide a highly specific detection of target analyte/molecule with artificial complementary scaffolds in the polymeric nanostructures relay with tunable aspect ratio, low cost synthesis procedure and applicability in harsh environment. To date, no molecular imprinted nanoparticles has been integrated with optical microwire platform in the literature. Here, we report the synthesis of a molecularly imprinted nanocarrier using hydrothermal process that act as receptors and combines optical microwire as transducing support. The detailed sensing process for one of the widely used pesticides (parathion methyl) in the detection range of 10−12 to 10−4 M with hyper-sensitivity and detection limit of 1.30 × 1012 nm/M and 79.43 fM respectively have been achieved. The compact sensing probe tested with real water samples collected from various sources show percentage recovery of around 100%. We strongly believe that the process for probe development will open a new gateway for next generation selective biosensing for biomedical research applications.
•First report, where molecular imprinted nanoparticles has been integrated with optical microwire platform for sensing.•We report molecularly imprinted nanocarrier as receptors in combination with optical microwire as transducing support.•Hyper-sensitivity and detection limit of 1.30 × 1012 nm/M and 79.43 fM respectively have been achieved with high selectivity.•The compact sensing probe tested with real water samples collected from various sources show very high percentage recovery.