HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation

• Published in: Journal of molecular biology Vol. 429; no. 19; pp. 2841 - 2858
• Main Authors: Tolbert, Michele, Morgan, Christopher E., Pollum, Marvin, Crespo-Hernández, Carlos E., Li, Mei-Ling, Brewer, Gary, Tolbert, Blanton S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 15-09-2017; Elsevier
• Subjects: BASIC BIOLOGICAL SCIENCES; enterovirus; Enterovirus A, Human - genetics; HeLa Cells; Heterogeneous Nuclear Ribonucleoprotein A1; Heterogeneous-Nuclear Ribonucleoprotein Group A-B - metabolism; hnRNP A1; Host-Pathogen Interactions; Humans; IRES; Magnetic Resonance Spectroscopy; NMR spectroscopy; Nucleic Acid Conformation; Protein Biosynthesis; Ribosomes - metabolism; RNA, Messenger - chemistry; RNA, Messenger - metabolism; SAXS Thermodynamics; Scattering, Small Angle; eIF; enterovirus; TOCSY; RDC; TROSY-HSQC; 5′UTR; SLII; CSP; 2AP; NOE; SAXS Thermodynamics; hnRNP A1; rNTP; RLuc; SAXS; SEC; EV; ITAF; AS; RD; FLuc; IRES; UP1; NOESY; NMR spectroscopy
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2017.06.007

Enteroviruses use a type I Internal Ribosome Entry Site (IRES) structure to facilitate protein synthesis and promote genome replication. Type I IRES elements require auxiliary host proteins to organize RNA structure for 40S ribosomal subunit assembly. Heterogeneous nuclear ribonucleoprotein A1 stimulates enterovirus 71 (EV71) translation in part through specific interactions with its stem loop II (SLII) IRES domain. Here, we determined a conjoined NMR-small angle x-ray scattering structure of the EV71 SLII domain and a mutant that significantly attenuates viral replication by abrogating hnRNP A1 interactions. Native SLII adopts a locally compact structure wherein stacking interactions in a conserved 5′-AUAGC-3′ bulge preorganize the adjacent helices at nearly orthogonal orientations. Mutating the bulge sequence to 5′-ACCCC-3′ ablates base stacking in the loop and globally reorients the SLII structure. Biophysical titrations reveal that the 5′-AUAGC-3′ bulge undergoes a conformational change to assemble a functional hnRNP A1–RNA complex. Importantly, IRES mutations that delete the bulge impair viral translation and completely inhibit replication. Thus, this work provides key details into how an EV71 IRES structure adapts to hijack a cellular protein, and it suggests that the SLII domain is a potential target for antiviral therapy.
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•The SLII IRES domain adopts a compact structure in solution wherein the high-affinity hnRNP A1 binding site is sequestered in a bulge loop.•The overall structure of SLII is determined by the sequence of its bulge loop since mutation leads to a global structural rearrangement.•A large negative heat capacity change is a thermodynamic signature of specific hnRNP A1–SLII recruitment.•Binding of hnRNP A1 induces a change in the conformation of the SLII bulge structure.