Characterization of the non-sulphated highly anionic Kurloff cell glycoconjugates by lectin- and immunoblotting: evidence for the presence of alpha (2,8)-linked polysialic acid-bearing molecules

Urea or guanidine hydrochloride-soluble extracts from highly purified Kurloff cells (KC) radiolabelled in vitro were subjected to DEAE-cellulose chromatography. Among the three anionic peaks obtained, a major and non-sulphated peak (designated as peak IV) strongly affected by glucosamine-labelling a...

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Bibliographic Details

Published in:	Biology of the cell Vol. 77; no. 3; p. 269
Main Authors:	Letaïef, S E, Landemore, G, Oulhaj, N, Izard, J
Format:	Journal Article
Language:	English
Published:	England 1993
Subjects:	Animals Anions Carbohydrate Conformation Galanthus Glycoconjugates - analysis Guinea Pigs Immunoblotting In Vitro Techniques Killer Cells, Natural - chemistry Lectins - metabolism Plant Lectins Polysaccharides, Bacterial - analysis Protein Binding Sialic Acids - analysis Sulfates - analysis
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