Damage to rabbit femoral articular cartilage following direct impacts of uniform stresses: an in vitro study

• Published in: Clinical biomechanics (Bristol) Vol. 14; no. 8; pp. 543 - 548
• Main Authors: Zhang, H., Vrahas, M.S., Baratta, R.V., Rosler, D.M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-1999
• Subjects: Animals; Arthritis - pathology; Biomedical engineering; Bone; Cartilage; Cartilage, Articular - injuries; Cartilage, Articular - pathology; Diseases; Impact; Kinematics; Post-traumatic arthritis; Rabbits; Stress, Mechanical; Wounds and Injuries - pathology; Impact; Post-traumatic arthritis; Cartilage
• ISSN: 0268-0033; 1879-1271
• DOI: 10.1016/S0268-0033(99)00010-8

Objective. To determine the acute gross and histologic damage resulting to femoral cartilage from an in vitro direct impact of uniform stress.
Design. Gross and histologic evaluations were performed on rabbit femoral condyles impacted by a drop-tower device.
Background. It is thought that impacts above a given threshold stress may initiate post-traumatic arthritis. The extent of damage following impacts of specific stress has not been previously studied.
Methods. 12 New Zealand White rabbit medial femoral condyles were divided into three groups by impact type and magnitude. A drop tower was used to strike the femoral condyle with a flat impactor, or with a custom contoured impactor. Gross and histological grades were given depending on the depth and number of fissures and cracks in the impacted condyle.
Results. The degree of damage correlated best with the type of impactor used and with the impact force; correlation between damage and impact stress was less significant. Contoured impactors tended to produce superficial fibrillation, while flat impactors tended to produce deep cracks. Impact forces above 500 N tended to create more severe damage than impact forces below 500 N. Subchondral bone remained intact in all cases and deep cartilage damage did not occur without disruption of more superficial layers. Poor correlation was found between damage as graded by gross examination versus damage graded histologically.
Conclusions. Acute damage corresponds best with type of impactor and impact force, and not as well with impact stress. Micro structural injuries may be present in the absence of gross findings.
Relevance
Post-traumatic arthritis is a disabling disease thought to occur when a blow of stress above a given threshold is delivered to articular cartilage. Current animal models of post-traumatic arthritis are unable to characterize the impact stress applied to an articular surface. This study examines grossly and histologically the structural damage occurring as a result of impacts of given stress and force.