Verification of the BALB/c 3T3 cell transformation assay after improvement by using an ITES-medium
We carried out the first-step verification study on our ITES-medium-improved BALB/c 3T3 cell transformation assay. In order to estimate its potential use as a short-term screening method for putative carcinogens, 31 chemicals were tested in the improved transformation assay. The test chemicals consi...
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| Published in: | Toxicology in vitro Vol. 17; no. 4; pp. 489 - 496 |
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01-08-2003
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| Abstract | We carried out the first-step verification study on our ITES-medium-improved BALB/c 3T3 cell transformation assay. In order to estimate its potential use as a short-term screening method for putative carcinogens, 31 chemicals were tested in the improved transformation assay. The test chemicals consisted of 18 carcinogens and 13 noncarcinogens. The present improved transformation assay did not use an exogenous metabolizing system. Data analysis was carried out on 34 chemicals, including assay data for three chemicals reported previously by the authors. As a result, the improved transformation assay showed a concordance of 73.5% with a rodent bioassay, a sensitivity for carcinogens of 71.4%, and a specificity for detection of noncarcinogens of 76.9%. The improved transformation assay detected all of the genotoxic carcinogens, and five of 11 nongenotoxic carcinogens as positive. It can be expected that the improved transformation assay will be able to detect not only genotoxic carcinogens with high probability but also approximately 50% of nongenotoxic carcinogens within about 3 weeks. Hence, these preliminary findings suggest that our improved transformation assay will be a reliable and useful short-term test procedure of screening for potential carcinogens, and it encourages us to conduct further experiments on many carcinogens and noncarcinogens. |
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| AbstractList | We carried out the first-step verification study on our ITES-medium-improved BALB/c 3T3 cell transformation assay. In order to estimate its potential use as a short-term screening method for putative carcinogens, 31 chemicals were tested in the improved transformation assay. The test chemicals consisted of 18 carcinogens and 13 noncarcinogens. The present improved transformation assay did not use an exogenous metabolizing system. Data analysis was carried out on 34 chemicals, including assay data for three chemicals reported previously by the authors. As a result, the improved transformation assay showed a concordance of 73.5% with a rodent bioassay, a sensitivity for carcinogens of 71.4%, and a specificity for detection of noncarcinogens of 76.9%. The improved transformation assay detected all of the genotoxic carcinogens, and five of 11 nongenotoxic carcinogens as positive. It can be expected that the improved transformation assay will be able to detect not only genotoxic carcinogens with high probability but also approximately 50% of nongenotoxic carcinogens within about 3 weeks. Hence, these preliminary findings suggest that our improved transformation assay will be a reliable and useful short-term test procedure of screening for potential carcinogens, and it encourages us to conduct further experiments on many carcinogens and noncarcinogens. |
| Author | Ajimi, Syozo Kajiwara, Yoshitsugu |
| Author_xml | – sequence: 1 givenname: Yoshitsugu surname: Kajiwara fullname: Kajiwara, Yoshitsugu email: kajiwara-yoshitsugu@ceri.jp organization: Chemicals Assessment Center, Chemicals Evaluation and Research Institute, Japan, 3-822, Ishii-machi, Hita-shi, Oita 877-0061, Japan – sequence: 2 givenname: Syozo surname: Ajimi fullname: Ajimi, Syozo organization: Hita Laboratory, Chemicals Evaluation and Research Institute, Japan, 3-822, Ishii-machi, Hita-shi, Oita 877-0061, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/12849733$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1093_mutage_gew010 crossref_primary_10_1080_15376520590945667 crossref_primary_10_1093_mutage_geu013 crossref_primary_10_1155_2011_935160 crossref_primary_10_1177_026119290803600609 crossref_primary_10_1016_S1665_2681_19_31371_7 crossref_primary_10_1177_026119291003800111 crossref_primary_10_1016_j_mrgentox_2011_11_009 crossref_primary_10_5487_TR_2008_24_1_037 crossref_primary_10_1007_s11356_018_1396_5 crossref_primary_10_1186_s12885_022_10053_0 |
| Cites_doi | 10.1016/0304-3835(85)90096-5 10.1289/ehp.9610440 10.2307/3431405 10.1126/science.6280280 10.1002/em.2850160503 10.1093/carcin/8.6.803 10.1016/0027-5107(85)90052-1 10.1016/0027-5107(77)90177-4 10.1016/S0027-5107(99)00216-X 10.1016/0027-5107(95)00241-3 10.1002/ijc.2910120217 10.1016/0165-1110(83)90009-X 10.1002/(SICI)1097-0215(19971009)73:2<271::AID-IJC18>3.0.CO;2-I 10.1093/mutage/3.4.355 10.1016/S1383-5718(97)00088-0 10.1016/0165-1110(91)90003-E 10.1093/mutage/4.4.286 10.1016/0165-1110(83)90036-2 10.1016/0027-5107(89)90172-3 10.1002/em.2850160502 10.1093/carcin/12.7.1287 10.1289/ehp.93100307 10.1093/carcin/16.8.1887 |
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| Keywords | BALB/c 3T3 cells Nongenotoxic carcinogens ITES-medium Improved cell transformation assay EPA |
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| SubjectTerms | 3T3 Cells Animals BALB/c 3T3 cells Carcinogenicity Tests - methods Carcinogens - toxicity Cell Transformation, Neoplastic - drug effects Culture Media - chemistry Dose-Response Relationship, Drug Improved cell transformation assay ITES-medium Mice Mice, Inbred BALB C Nongenotoxic carcinogens Reproducibility of Results |
| Title | Verification of the BALB/c 3T3 cell transformation assay after improvement by using an ITES-medium |
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